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Effects of aspiration follicular pressure in the oocytes class I and II recovered



Effects of aspiration follicular pressure in the oocytes class I and II recovered



Revista Brasileira de Reproducao Animal 26(3): 147-149, Jul -Set



The objective of the present work was to determinate the follicular aspiration pressure to obtain the best quality and quantitative ratio of recovered oocytes. The recovered oocytes were classified according to the number of granulosa cell layers and citoplasm homogenity as class I, II, III, expanded and degenered or naked, being consedered viable oocytes those from class I and II. The oocytes were obtained from ovaries of slaughterhouse animals. Only follicles of 2-8 mm of diameter were aspirated, using a 18G X 1 1/2 needle (40 X 12) in a 50 ml conic tube with a vaccum pump, with different aspirations pressures 50, 75, 90, 100, 120 mmHg. The results obtained was: pressure of 50 mmHg recovered 49 viable oocytes from class I (12,2 +- 7,0 b), and 39 from class II (9,7 +- 3,3 b), the pressure of 75 mmHg recovered 53 viable oocytes from class I (13,2 +- 5,0 b) and 56 from class II (14,0 +- 6,7 b), pressure of 90 mmHg recovered 44 viable oocytes from class I (11,0 +- 4,7 b) and 34 from class II (8,5 +- 3,8 b), pressure of 100 mmHg recovered 103 viable oocytes from class I (25,7 +- 12,5 a) and 123 from class II (30,7 +- 15,6 a) and the pressure of 120mmHg recovered 24 viable oocytes from class I (6,0 +- 4,2 b) and 37 from class II (9,2 +- 5,0 b).

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Accession: 034815243

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Effects of follicular aspiration pressure on the class I and II oocytes recovered. Revista Brasileira de Reproducao Animal 26(3): 147-149, 2002

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