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Erythropoietin stimulates neurogenesis in adult rat brain and organotypic hippocampal slice cultures



Erythropoietin stimulates neurogenesis in adult rat brain and organotypic hippocampal slice cultures



FASEB Journal 18(4-5): Abst 311 26



Adult neurogenesis occurs in the rostral subventricular zone of the lateral ventricles and the subgranular zone of the dentate gyrus in the hippocampus. Erythropoietin (EPO) is a trophic factor to promote proliferation and differentiation of erythroid progenitor cells. This study demonstrates effect of EPO on neurogenesis of the adult rat brain. EPO was treated by intraventricular infusion (40 Unit, once a day for 3 days) in the lateral ventricle of the adult rats. Significant increase of BrdU-positive cells in SVZ resulted in EPO treated brain with respect to aCSF treated brain. These BrdU-positive cells were identified as neuron cells by using double labeling with NeuN. Effect of EPO on neurogenesis was demonstrated also in the organotypic hippocampal slice cultures of the neonatal rat brains. Under normal condition of slice culture, EPO treatment produced a significant increase of BrdU-positive cells in DG (all of 0.4, 4, and 40 U/ml) and CA1 (only at 40 U/ml) regions. Oxygen/glucose deprivation (for 45 min) reduced BrdU-positive cells significantly in the CA1. EPO treatment (40 U/ml) under OGD produced significant increase of BrdU-positive cells in the CA1 (vulnerable zone to ischemia) and DG (proliferative zone to ischemia). These results suggest that EPO has an important role in adult neurogenesis and in neurorepair of damaged brain. This study was supported by Brain Korea 21 project of Korea government.

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