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Estimation of the number of polyhydroxyalkanoate -degraders in soil and isolation of degraders based on the method of most probable number using PHA-film



Estimation of the number of polyhydroxyalkanoate -degraders in soil and isolation of degraders based on the method of most probable number using PHA-film



Bioscience Biotechnology & Biochemistry 65(5): 1214-1217



A new method to estimate the number of polyhydroxyalkanoates (PHA)-degraders in soil and to isolate degraders, called the film-MPN method, is proposed. The incubation time was measured by the first order reaction (FOR) model. This method was used to estimate numbers of poly (3-hydroxybutyrate-co-3-hydroxyvalerate)(P(3HB-co-3HV))- and poly(3-hydroxyvalerate-co-4-hydroxybutyrate)(P(3HB-co-4HB))-degraders in garden soil (4.30X105 and 2.15X105 aerobic degraders per gram of dry soil, respectively). The number of P(3HB-co-HV)-degraders in paddy field soil was 5.06X105 aerobic degraders per gram dry soil. Also, several P(3HB-co-3HV)-degraders were isolated directly from positive-growth tubes of high dilution.

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