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Estrogen influences in neurogenesis zones after focal cerebral ischemia


, : Estrogen influences in neurogenesis zones after focal cerebral ischemia. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 392 7

Neurogenesis in the adult mammalian brain is limited to two regions, (1) the subventricular zone(SVZ) and (2) the subgranular zone(SGZ) of the hippocampal dentate gyrus. Recent reports suggest that focal cerebral ischemia(FCI) enhances neurogenesis. Our laboratory has shown that estradiol is able to protect against FCI. The current study explored the effects of estradiol on proliferative zones of the adult mouse brain after permanent middle cerebral artery occlusion(MCAO). Adult mice were ovariectomized and implanted with silastic capsules containing oil(vehicle) or a low-physiological dose of 17beta-estradiol(E2). One week later, the animals underwent permanent MCAO. Beginning the morning prior to MCAO, animals received BrdU (50mg/kg, ip inj.) twice daily for 4 days. The animals were perfused 96h after the initiation of MCAO. Coronal sections of the brains were stained via immunohistochemistry (IHC) for BrdU. BrdU positive (BrdU+) cells were counted. In the most dorsal region of the SVZ, we observe a striking increase in BrdU+ cells in the estradiol-treated compared to oil-treated animals. This effect is not observed in the more ventral regions of the SVZ. In the SGZ, there appear to be fewer BrdU+ cells in estradiol treated animals. Surprisingly, none of the zones analyzed showed significant ipsilateral vs. contralateral differences in BrdU+ cells. We are currently pursuing double-label IHC studies to elucidate the identities of proliferating/differentiating cells in these regions. Our data demonstrate regionally specific effects of estradiol on cellular proliferation in the injured brain.

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