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Evaluation of BacT/ALERT charcoal-containing media in plastic blood culture bottles



Evaluation of BacT/ALERT charcoal-containing media in plastic blood culture bottles



Abstracts of the General Meeting of the American Society for Microbiology 101: 147



Breakage of glass blood culture bottles can pose significant safety issues through potential contact with blood-borne pathogens. The current glass BacT/ALERT culture bottles are enclosed in a thin film of plastic to contain potential spills in the rare event that breakage occurs. In a continuing effort to improve workplace safety, Organon Teknika Corporation has developed a lighter, break-resistant plastic blood culture bottle. Three charcoal-containing blood culture bottles have recently been released to the marketplace in standard glass bottles - BacT/ALERT FA for aerobic culture, BacT/ALERT FN for anaerobic culture, and BacT/ALERT PF for culture of pediatric specimens. These products represent a new generation of non-vent BacT/ALERT media with enhanced performance characteristics. In this study, these three media types were filled in both glass and plastic bottles, and evaluated to determine recovery and detection times of low levels of various microorganisms diluted in human blood. Detection times in plastic BacT/ALERT FA, BacT/ALERT FN, and BacT/ALERT PF culture bottles inoculated with 11-100 CFU were equivalent to the existing glass bottles, and all inoculated bottles were positive. For BacT/ALERT FA at a 1-10 CFU inoculum, detection times ranged from 1.8 hours quicker to 5.5 hours slower in plastic bottles, and 65/66 inoculated plastic bottles were positive (98%), compared to 63/66 glass bottles (95%). For BacT/ALERT FN at a 1-10 CFU inoculum, detection times ranged from 10.4 hours quicker to 0.4 hours slower in plastic bottles, and 65/66 inoculated plastic bottles and glass bottles were positive (98%). For BacT/ALERT PF at a 1-10 CFU inoculum, detection times were identical in both bottle types, and 59/66 inoculated plastic bottles were positive (89%), compared to 58/66 glass bottles (88%). We conclude that plastic BacT/ALERT charcoal-containing culture bottles exhibit equivalent performance to the existing glass culture bottles, and provide a safer and more convenient product to the customer. The plastic bottles are currently undergoing clinical evaluation.

Accession: 034878115

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