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Follicular dynamic, ovulation number and embryo recovery in mares treated with equine pituitary extract and equine purify FSH



Follicular dynamic, ovulation number and embryo recovery in mares treated with equine pituitary extract and equine purify FSH



Revista Brasileira de Reproducao Animal 27(3): 506-508, Jul -Set



Several methods have been study to obtain a good superovulation answer and good embryo recovery rates in mares. Equine pituitary extract (EPE) is used to induce multi ovulation in mares, but the results are lower than other species. The experiment was conducted to compare the efficacy of two different treatments, the EPE and equine FSH in to promote superovulation in mares, to characterize the dynamic of follicular growth in the two different treatments. Were utilized six crossbreed mares, these animals were evaluated by ultrasonography, for the ovulation detection, and divided in tree alleatory groups: Group 1 (control), Group 2 (application EPE 25mg/IM, twice daily), and Group 3 (application FSH equine 12mg/IM, twice daily). Follicular growth and ovulation were monitored daily by transrectal palpation and ultrasonography. Upon detection of a follicle >= 35mm, Group 1 mares were administered 2500UI hCG and inseminated with 1X109 viable spermatozoa. For mares in Group 2 and 3 the treatment was discontinued when the majority of follicles in growth reached a diameter of >=35mm and administered 3000UI hCG. The insemination were performed as described for group 1 every other day until the last ovulation had occurred. The results shown that the EPE and the FSH equine improve superovulation in mares (3,4 vs 4,2) and improve the number embryos recovery (1,0 vs 2,2), when compared the control group (0,4 embryos/mare) (P<0,05). No statistics differences were found in the follicular diameter growth rates (mm/day) and day of treatment between the groups. Although, the mean of follicle pre ovulatory diameter are significantly higher (P<0,05) for the G1 (41.1+-4,2) than G2 (34,6+- 4,6); G3 had value intermediary between the others groups and no statistic differences was found. The number of days from PGF2alpha application to ovulations was higher (P<0,05) for G2 (11,0+-2,2) than G1 (9,0+-0,6) and similar G3. Therefore, the two treatment induce satisfactory superovulatory response when compared with non treated group. These treatments could be used to improve the efficacy of equine embryo transfer programs.

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Accession: 034945525

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