+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Fractional disinfection procedure for Huperzia lucidulum explants

, : Fractional disinfection procedure for Huperzia lucidulum explants. Hortscience 36(3): 583-584, June

The shining club moss, Huperzia lucidulum, can be propagated in tissue culture, but it is difficult to obtain sterile explants. To facilitate surface sterlization of H. lucidulum, a fractional disinfection procedure was developed. Based on the principle that vegetative microbial cells are more easily destroyed than spores, fractional disinfection induces spore germination before the treatment of explants with a sodium hypochlorite solution. This study compares the effectiveness of fractional disinfection to standard disinfection methods for club moss explants. When standard methods were used, 100% of H. lucidulum apical tips disinfected for 10, 20, or 30 minutes with 1% sodium hypochlorite/0.15% Tween 20 either quickly showed evidence of microbial contamination or turned brown. In contrast, when one 24 h cycle of fractional disinfection was used to surface sterilize H. lucidulum explants, 22% remained contamination free for over 5 weeks. Even better, when club moss explants were treated with two consecutive 24 h cycles of fractional disinfection, 50% remained contamination free for over five weeks. Because the fractional disinfection procedure was more effective than standard disinfection methods, it was applied to the original club moss explants, 81% of which had remained contaminated even after multiple standard disinfections. Applying the fractional disinfection method to these contaminated cultures produced an additional 15.6% contamination free club moss apical tips. After successful surface sterilization, H. lucidulum apical tips and gemmae established in vitro grew normally compared to conventionally propagated club moss cuttings and gemmae in nature.

Accession: 034951186

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Dwyer, T.; Waegel, A.S., 2004: Effect of growth regulators on Huperzia lucidulum explants in vitro. In vitro, Huperzia lucidulum shoot tips grow slowly and do not readily form roots, although gemmae produce roots within 4-6 weeks. Experiments were designed to determine if shoot growth and root formation could be accelerated with the growth regul...

Salehi, H.; Khosh-Khui, M., 1997: A simple procedure for disinfection of 'Baby Masquerade' miniature rose explants. Explants of a miniature rose (Rosa chinensis Jacq. var. minima Rehd hybrids) cultivar 'Baby Masquerade' with internal contaminants were used in this study to find the most suitable procedure for their disinfection. Single-node explants w...

Konrath, E.L.; Neves, B.M.; Passos, C.Dos.S.; Lunardi, P.S.; Ortega, M.G.; Cabrera, J.L.; Gonçalves, C.A.; Henriques, A.T., 2013: Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain. Huperzine A, a <italic>Lycopodium</italic> alkaloid produced by Chinese folk herb <italic>Huperzia serrata</italic> (Lycopodiaceae), has been shown to be a promising agent for the treatment of Alzheimer's disease due t...

Borneff, M.; Pichl, R., 1989: Disinfection in dental practice. First test results of a new disinfection procedure for dental impression materials. Zwr 98(4): 358-361

Park, G-Hun.; Chang, S.Eun.; Bang, S.; Won, K.Hee.; Won, C.Hyun.; Lee, M.Woo.; Choi, J.Ho.; Moon, K.Chan., 2015: Usefulness of Skin Explants for Histologic Analysis after Fractional Photothermolysis. Fractional laser resurfacing treatment has been extensively investigated and is widely used. However, the mechanism underlying its effects is poorly understood because of the ethical and cosmetic problems of obtaining skin biopsies required to stu...

Gonzalez, O.; Silva, J.; Meneses, S., 1997: Disinfection of sweet potato explants (Ipomoea batatas (L.) Lam.). Sodium hypochlorite (0.1-2,0% W/V), calcium hypochlorite (3.0-9.0% W/V) and mercury bichloride (0.1-0.9% W/V) were used for disinfecting leaf explants of sweet potato variety CEMSA 78'-354. The best control of pathogenic fungi and bacteria wa...

Valicek, P.; Wahaishi, A.K., 1989: Contamination of explants of Coffea arabica L. as depending on the disinfection used. Six combinations of disinfectants were tested for different exposure times in coffee meristem culture on 3 modifications of LS 65 medium. The lowest av. contamination was recorded with 10% Savo (sodium hypochlorite) + 0.2 ml Tween 20. After 25, 30...

Medeiros, C.P.C. de; Correia, D.; Luz, J.M.Q.; Rosseti, A.G.; Benbadis, A., 2000: In vitro culture of nodal explants of hog plum (Spondias mombin L.): disinfection stage. S. mombin shoots previously treated with benomyl (2.0 mg/litre) and washed with running water and detergent were subjected to 4 disinfection treatments: (1) 1% (v/v) sodium hypochlorite, (2) 70% ethanol, 1% (v/v) sodium hypochlorite, (3) 70% ethan...

Arteaga Amador, M.; Pena Garcia, E.; Perez Montesino, D.; Torriente Campos, Z.; Kang CholGyu, 1998: Disinfection of Hippeastrum vittatum explants as a determining factor for large scale propagation with commercial aims in Cuba. Nine sterilizing procedures and 7 culture media were compared for culture initiation from inner and outer bulb scales of H. vittatum (cv. Equestre Red). Dipping bulbs in boiling water for 2 or 3 minutes prior to a series of steps involving chemica...

Helbig, D.; Mobius, A.; Simon, J.C.; Paasch, U., 2011: Heat shock protein 70 expression patterns in dermal explants in response to ablative fractional phothothermolysis, microneedle, or scalpel wounding. Wounds 23(3): 59-67