Gene cloning, sequence analysis and prokaryotic expression vector construction of duck MHC-I alpha chain mature peptide of the extracellular domain

SunKai; LiXinSheng; CuiBaoAn; ChenHongYing; WeiZhanYong; ZhangLei; SongYaPeng; RuanWuYing

Journal Of Northwest A and F University: 2, 57-62

2010


Accession: 034974343

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 1 workday
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
Objective: The study was done to clone duck MHC-I alpha chain mature peptide gene cDNA sequence of the extracellular domain and construct a prokaryotic expression plasmid. Method: The cDNA of duck MHC-I alpha chain extracellular domain of mature peptide gene amplified with RT-PCR from healthy duck spleen tissue was cloned into pGEM-T Easy vector by T/A Ligation.