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Genetic complementation analysis of uranium reduction-deficient mutants of Shewanella putrefaciens



Genetic complementation analysis of uranium reduction-deficient mutants of Shewanella putrefaciens



Abstracts of the General Meeting of the American Society for Microbiology 101: 610



Shewanella putrefaciens strain 200, a non-fermenting member of the gamma-proteobacteria, is capable of respiring anaerobically on a variety of compounds as sole terminal electron acceptor including ferric iron, nitrate, nitrite, manganese dioxide, sulfite, thiosulfate, TMAO, and fumarate. Anaerobic growth experiments on defined minimal media demonstrated that S. putrefaciens can also utilize uranyl carbonate (U(VI)) as an alternate electron acceptor while oxidizing lactate as sole carbon/energy source. A rapid plate assay screening technique has facilitated the isolation of a set of ethyl methane sulfonate-generated U(VI) reduction-deficient mutants. Mutant subclasses deficient in anaerobic growth on U(VI) and alternate terminal electron acceptors have been identified and characterized via genetic complementation analysis.

Accession: 034981777

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