+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Helicobacter pylori induces dendritic cell maturation and th1 cytokine production



Helicobacter pylori induces dendritic cell maturation and th1 cytokine production



Digestive Disease Week Abstracts & Itinerary Planner : Abstract No W910



Background: Helicobacter pylori (HP) is an extracelluar organism that resides in the mucous layer of the gastric epithelium. Evidence suggests that HP can induce gastric inflammation without invading mucosal cells. It is believed that HP binds to gastric epithelial cells and stimulates the secretion of inflammatory cytokines. Breakdown of the epithelial barrier ensues allowing translocation of HP bacterial antigens which further stimulates antigen presenting cells (APC), e.g., macrophages in the l amina propria. However, it is known that tissue dendritic cells are a more efficient APC. A recent study by Rescigno et al. reported the ability of intestinal dendritic cells (DCs) to sample the lumen for the presence of bacteria without disturbing the i ntegrity of the epithelial barrier (Nature Medicine 2:361, 2001). It is unclear whether a similar process can occur in the stomach and thus whether HP triggers its Th1 immune response by activating dendritic cells. Aims: The goal of this study was to d et ermine whether live HP can induce DC maturation and Th1 cytokine release. Methods: Seven-day old bone marrow-derived DCs were obtained from C57BL/6 mice and were co-cultured with PBS or 108 organisms/ml of actively proliferating HP for 24 h. D Cs co-cultured with PBS served as controls. DCs were dual-labeled with FITC-MHC II and PE-CD40, PE-IL-12 or PE-IL-10 and analyzed by FACS. CD40 is a surface antigen expressed on mature dendritic cells involved in T cell activation. The mean for two exp eriments performed in triplicate are shown +/- the range. Results: Surface staining revealed that HP induced a two-fold increase in the percent of DCs expressing CD40 (PBS=16.9 +/- 8.7% , HP=35.4 +/- 4.6%). Intracellular cytokine staining showed a signif icantly higher percent of DCs expressing IL-12 (PBS=2 +/- 0.06%, HP=26.1 +/- 0.4%). No significant difference was seen for IL-10 (PBS 1.5 +/- 0.13%, HP=2.8 +/- 0.45%). Conclusions: HP induces DC maturation and Th1 cytokine release. These findings implicate a potential role of DCs as the APC mediating HP-induced gastritis.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 035021558

Download citation: RISBibTeXText

DOI: 10.1016/S0016-5085(03)83001-9


Related references

Helicobacter pylori induces human dendritic cell maturation and duodenal ulcer promoting gene a (dupA) enhances dendritic cell cytokine expression. Gut 60(Suppl 1): A54-A54, 2011

Helicobacter pylori outer membrane protein 18 (Hp1125) induces dendritic cell maturation and function. Helicobacter 10(5): 424-432, 2005

IRAK-M expression limits dendritic cell activation and proinflammatory cytokine production in response to Helicobacter pylori. Plos One 8(6): E66914, 2014

Induction of maturation and cytokine release of human dendritic cells by Helicobacter pylori. Infection and Immunity 72(8): 4416-4423, 2004

Mycobacterial heat shock protein 70 induces interleukin-10 production: immunomodulation of synovial cell cytokine profile and dendritic cell maturation. Clinical and Experimental Immunology 135(2): 336-342, 2004

Helicobacter pylori promotes the production of thymic stromal lymphopoietin by gastric epithelial cells and induces dendritic cell-mediated inflammatory Th2 responses. Infection and Immunity 78(1): 108-114, 2010

Helicobacter pylori urease induces monocyte/macrophage activation and cytokine production. Gastroenterology 108(4 SUPPL ): A109, 1995

Interferon gamma induces maturation of the dendritic cells which infiltrate into the pyloric mucosa of mongolian gerbil treated with Helicobacter pylori and N-methyl-N-nitro-N-nitrosoguanidine. Gastroenterology 118(4 Suppl 2 Part 1): AGA A762, 2000

Campylobacter jejuni induces maturation and cytokine production in human dendritic cells. Infection and Immunity 74(5): 2697-2705, 2006

Dendritic-cell-associated C-type lectin 2 (DCAL-2) alters dendritic-cell maturation and cytokine production. Blood 107(4): 1459-1467, 2005

Helicobacter pylori heat-shock protein 60 induces production of the pro-inflammatory cytokine IL8 in monocytic cells. Journal of Medical Microbiology 54(Pt 3): 225-233, 2005

Leptospira interrogans is recognized through DC-SIGN and induces maturation and cytokine production by human dendritic cells. Fems Immunology and Medical Microbiology 53(3): 359-367, 2008

Helicobacter pylori DNA decreases pro-inflammatory cytokine production by dendritic cells and attenuates dextran sodium sulphate-induced colitis. Gut 60(11): 1479-1486, 2012

Allogeneic reaction induces dendritic cell maturation through proinflammatory cytokine secretion. Transplantation 77(2): 267-275, 2004

Helicobacter pylori cytotoxin-associated gene A impairs human dendritic cell maturation and function through IL-10-mediated activation of STAT3. Journal of Immunology 192(1): 316-323, 2014