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Induction of tyrosine hydroxylase in cultured neurons of the accessory olfactory bulb by coculture with dissociated vomeronasal cells

Induction of tyrosine hydroxylase in cultured neurons of the accessory olfactory bulb by coculture with dissociated vomeronasal cells

Society for Neuroscience Abstracts 27(1): 367

We have established the culture system of the accessory olfactory bulb (AOB) to investigate functional roles of each neuron in pheromonal signal processing (Muramoto et al, Soc.Neurosci.Abstr. 25, 1278, 1999). Furthermore, we have tried to develop the coculture system of the cultured AOB neurons with the dissociated cells of vomeronasal organ (VNO). The dissociated VNO cells aggregate each other and form spherical structures with central cavity, called vomeronasal pockets (VP). In the main olfactory bulb (MOB), the projection and activities from olfactory receptor neurons affects cell differentiation and maturation in the MOB. It was also reported that the expression of tyrosine hydroxylase (TH) in MOB culture was induced by coculture with the explants of olfactory epithelium. Thus, we investigated effects of VNO cells on the AOB neurons. At 1 day in culture, some VPs were transferred on the AOB culture system, and the number of TH-immunopositive neurons was counted after 14 days of beginning of coculture. TH-positive neurons in AOB culture were significantly increased by coculture, while no significant changes were observed in coculture between MOB and VP. These results suggest that the differentiation and/or maturation of AOB neurons are also modified by VNO neurons through the similar mechanisms of MOB.

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