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L-DOPA, Ropinirole and Bromocriptine have a Differential Effect on Srtiatal Glutamic Acid Decarboxylase 65 and 67 mRNA Expression in MPTP-treated Marmosets



L-DOPA, Ropinirole and Bromocriptine have a Differential Effect on Srtiatal Glutamic Acid Decarboxylase 65 and 67 mRNA Expression in MPTP-treated Marmosets



FASEB Journal 18(4-5): Abst 398 3



Glutamic acid decarboxylase (GAD) is the synthetic enzyme for GABA and exists as two distinct isoforms GAD65 and GAD67. Expression of these two isoforms may reflect different functional state of GABAergic neurones in the basal ganglia and may play an important role in Parkinson&39;s disease (PD). We now report the effect of repeated oral treatment of MPTP-treated common marmosets with L-DOPA plus carbidopa (12.5 mg/kg plus 12.5 mg/kg), bromocriptine (0.5 mg/kg) and ropinirole (0.3 mg/kg) for 28 days on GAD65 and GAD67 mRNAs expression in the brain slices using in situ hybridisation histochemistry. MPTP treatment increased both GAD65 and GAD67 mRNA expression in caudate nucleus and putamen (p<0.05, Newman Keul&39;s test). L-DOPA caused a further increase in GAD65 mRNA expression (p<0.01) while reversed the increase in GAD67 mRNA expression (p<0.05) compared to MPTP treatment. Bromocriptine administration did not reverse the increase in both GAD65 and GAD67 mRNA expression in the areas measured. However ropinirole reversed the increase in GAD65 and GAD67 mRNA expression (p<0.05). The differential alterations in striatal GAD65 and GAD67 mRNA levels induced by L-DOPA administration may explain the motor complications occuring on long term treatment of PD.This study is supported by National Parkinson&39;s Foundation USA and Parkinson&39;s Disease Society UK.

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Accession: 035201310

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