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Laser - induced exocytosis in PC - 12 cells is triggered by calcium release from internal stores



Laser - induced exocytosis in PC - 12 cells is triggered by calcium release from internal stores



Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 581 9



Calcium-dependent exocytosis in PC12 cells can be triggered by illumination of the plasma membrane and the immediate intracellular space by an evanescent field from an argon laser on a TIRF microscope setup. We imaged single secretory granules which were labeled with acridine orange, a marker of acidic membrane compartments. Turbulent exocytotic activity starts after a delay of a few tens of seconds and lasts for up to two minutes. This activity does only partially depend on external calcium, but it is suppressed completely by additional administration of ryanodine, a specific blocker of calcium release from intracellular stores. We analyzed the frequency, time course and spatial distribution of single exocytotic events. Exocytosis in PC12 cells occurs at specific sites, so called 'hot spots' and the duration of single secretion events varies considerably from tens of milliseconds up to a few seconds. We found that some cells had long lasting exocytotic events while others exhibited almost exclusively very short exocytotic flashes. These different kinetics may originate from two distinct release mechanisms, namely complete granule fusion and 'kiss and run' type of release which occurs through a long-living fusion pore.

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Accession: 035210123

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