+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Molecular characterization of genetically distinct strains of bean pod mottle virus

Molecular characterization of genetically distinct strains of bean pod mottle virus

Phytopathology 92(6 Suppl): S32

ISSN/ISBN: 0031-949X

BPMV (genus Comovirus), an economically important pathogen of soybean, has a positive sense single stranded bipartite RNA genome, designated RNA1 and RNA2. We have previously reported the occurrence in nature of two distinct subgroups of BPMV strains (subgroups I and II), as well as reassortants between the two subgroups, that can be clearly distinguished based on nucleic acid hybridization and nucleotide sequence analyses. Now we report on the production of infectious full-length cDNA clones of genomic RNAs 1 and 2 from representative strains of the two subgroups and reassortants. Interestingly, the reassortant strains induced severe to very severe symptoms on their soybean host plants, whereas strains in either subgroup I or II induced only mild or moderate symptoms. This phenomenon was also observed when pseudorecombinants were constructed in the laboratory from heterologous infectious transcripts. The availability of the infectious cDNA clones from genetically distinct strains makes it possible to generate the appropriate chimeric constructs that allow reaping the determinants of symptom severity.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 035329606

Download citation: RISBibTeXText

Related references

Biological and molecular characterization of four broad bean mottle virus strains. Phytopathology 81(10): 1243, 1991

Molecular Characterization of Naturally Occurring RNA1 Recombinants of the Comovirus Bean pod mottle virus. Phytopathology 97(10): 1255-1262, 2007

Molecular characterization of isolates of anagyris vein yellowing virus, plantago mottle virus and scrophularia mottle virus -- comparison of various approaches for tymovirus classification. Archives of Virology 150(11): 2325-2338, 2005

Molecular characterization of Sunflower chlorotic mottle virus: a member of a distinct species in the genus Potyvirus. Archives of Virology 155(8): 1331-1335, 2010

Analysis of complete genomic sequences of isolates of the Sweet potato feathery mottle virus strains C and EA: molecular evidence for two distinct potyvirus species and two P1 protein domains. Archives of Virology 155(12): 2059-2063, 2010

Molecular Characterization and Detection of a Genetically Distinct Tomato Chlorosis Virus Strain in Taiwan. Plant Disease 102(3): 600-607, 2018

Characterization of genetically distinct subgroup of Candida albicans strains isolated from oral cavities of patients infected with human immunodeficiency virus. Journal of Clinical Microbiology 33(3): 696-700, 1995

Some conditions affecting the self assembly of 3 spherical viruses abstract cowpea chlorotic mottle virus brome mosaic virus broad bean mottle virus. Phytopathology 58(8): 1053, 1968

Biotic, molecular, and phylogenetic characterization of bean calico mosaic virus, a distinct begomovirus species with affiliation in the squash leaf curl virus cluster. Phytopathology 89(4): 273-280, 1999

Radish mosaic virus, a crucifer virus serologically re lated to strains of bean pod mottle virus and to squash mosaic virus. Phytopathology 54(11): 1418-1424, 1964

Soybean green stem caused by selected strains of bean pod mottle virus. Phytopathology 94(6): S158-S158, 2004

Laboratory strains of murine cytomegalovirus are genetically similar to but phenotypically distinct from wild strains of virus. Journal of Virology 82(13): 6689-6696, 2008

The nucleotide sequence and genome organization of the RNA-1 segment in two bromoviruses: broad bean mottle virus and cowpea chlorotic mottle virus. Virology 185(2): 553-562, 1991

A Survey of Viruses Affecting French bean (Phaseolus vulgaris) in Iran Includes a First Report of Southern bean mosaic virus and Bean pod mottle virus. Plant Disease 89(9): 1012, 2005

A survey of viruses affecting French bean (Phaseolus vulgaris) in Iran includes a first report of Southern bean mosaic virus and Bean pod mottle virus. Plant Disease 89(9): 1012, 2005