Molecular cloning, expression vector construction and prokaryotic expression of BnCIo1 gene from Brassica napus

DingYong; ChangWei; LiuXiaoZhu

Scientia Agricultura Sinica: 2, 252-258

2010


Accession: 035331210

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Abstract
Objective: BnClo1 gene cloned from Brassica napus was expressed in Escherichia coli ER2566. Method: A cDNA fragment about 750 bp was amplified from the total RNA of rape seeds by reverse transcription PCR (RT-PCR) with a pair of specific primers based on the sequences of the BnCIo1 gene.