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Multi-factor differentiation of adult bone marrow stem cells along the neuronal pathway



Multi-factor differentiation of adult bone marrow stem cells along the neuronal pathway



FASEB Journal 16(4): A506, March 20



Degenerative diseases of nervous tissue and traumatic injuries to the brain and spinal cord affect millions of Americans each year. The recent discovery that pluripotent adult bone-marrow-derived stem cells (BMSC) can generate neuronal precursor cells in vitro holds promise for treatments of neural diseases and injuries involving the use of a patient's own cells. Various methods have been used to initiate neuronal differentiation of BMSC by employing certain factors such as epidermal growth factor (EGF) and brain-derived neurotrophic factor (BDNF). We demonstrated that a specific four-factor protocol employing 3-Isobutyl-1-methylxanthine (IBMX), butylated hydroxyanisole (BHA), nerve growth factor (NGF), and BDNF optimizes the neuronal differentiation of rat BMSC in culture. The cells exhibited identifiable neuronal morphologies and expressed specific neuronal markers including glial fibrillary acidic protein (GFAP), neuron specific enolase (NSE), and neurofilament.

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