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Multi-laboratory evaluation of procedures for processing of cord blood

Multi-laboratory evaluation of procedures for processing of cord blood

Blood 98(11 Part 2): 334b, November 16

Various procedures can be used to isolate stem and progenitor cells from cord blood. This study evaluated the traditional hydroxethyl starch sedimentation (HES) and top-bottom (T-B) procedures and stem and progenitor cell-capturing filter systems recently developed by two manufacturers, Asahi (filter A) and Terumo (filter B) Companies. Each of seven laboratories processed cord blood preparations chosen by randomization to one of the traditional methods or one of the filters (n=8 for both methods). In addition, the processed preparations were frozen. Each laboratory utilized routine traditional processing and testing procedures. With the filters the cells were removed by back-flushing. In vitro tests were performed with collected, processed and frozen-thawed samples. The results were expressed as the per cent recovery of cell parameters in processed versus collected samples (performance 1 (PF1)) and in thawed versus processed samples (performance 2 (PF2)). The composite results obtained by the seven laboratories are summarized. Total nucleated cell (TNC) PF1 levels were comparable for the two traditional procedures (median 79 and 86%) and reduced with both filters (median 58 and 61%). Mononuclear cell (MC) PF1 levels were highest with the T-B procedure (median 91%), reduced on using filter A (median 77%) and comparable with the HES and the filter B procedures (median levels 72 and 70%). CD34+ cell counts PF1 levels were comparable with the T-B and filter B procedures (median 96%) and reduced with the HES and filter A procedures (median 84 and 75%). A larger percentage of red cells and platelets were removed during processing with the filter procedures. With the HES and filter B procedures, the TNC and MC PF1 recoveries were reduced with preparations having relatively higher collection volumes. On the other hand, CD34+ PF1 recoveries with each of the procedures appear to be independent of volume. The PF2 recoveries for TNC, MC, CD34+ cells, and colony forming cells do not appear to be influenced by the specific processing procedure. Substantial inter-laboratory differences were observed in some instances, possibly because of the assay procedures that were utilized. These studies indicate that filters that capture stem and progenitor cells may be an appropriate methodology for processing cord blood collected for banking.

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Accession: 035349272

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