+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Multi-loci genomic analysis

Multi-loci genomic analysis

Official Gazette of the United States Patent & Trademark Office Patents 1244(1), Mar 6

The invention provides a method of sequencing a nucleic acid in a reaction mixture comprising first and second nucleic acid target sequences. The target sequences may be present on the same or different nucleic acid molecules. First and second labelled sequencing primers are provided that are hybridizable, respectively, to the first and second nucleic acid target sequences. The second sequencing primer being at least as long as the total length of the first sequencing primer plus the length of the first target sequence. Each of the sequencing primer extension products beginning with the second sequencing primer will be longer than the primer extension products in the pool beginning with the first sequencing primer. Once the sequencing reactions have been completed, the lengths of the primer extension products in the pool may be detected by known methods, such as by gel electrophoresis.

(PDF emailed within 1 workday: $29.90)

Accession: 035349314

Download citation: RISBibTeXText

Related references

Genomic DNA fingerprinting of Vitis vinifera by the use of multi-loci probes. Vitis 29(4): 223-227, 1990

Non-methylated Genomic Sites Coincidence Cloning (NGSCC): an approach to large scale analysis of hypomethylated CpG patterns at predetermined genomic loci. Molecular Genetics and Genomics 271(1): 22-32, 2003

Software for selecting the most informative sets of genomic loci for multi-target microbial typing. Bmc Bioinformatics 14(): 148-148, 2013

Linkage between loci of quantitative traits and marker loci: multi-trait analysis with a single marker. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 90(6): 776-786, 1995

Analysis of multiple genomic sequence alignments: a web resource, online tools, and lessons learned from analysis of mammalian SCL loci. Genome Research 14(2): 313-318, 2004

PHYLUCE is a software package for the analysis of conserved genomic loci. Bioinformatics 32(5): 786-788, 2015

Genomic analysis of Ovis aries (Ovar) MHC class IIa loci. Immunogenetics 60(3-4): 167-176, 2008

Analysis of interactions between genomic loci through Chromosome Conformation Capture (3C). Current Protocols in Cell Biology Chapter 22: Unit22.15-Unit22.15, 2013

Genomic analysis of the Tapasin gene, located close to the TAP loci in the MHC. European Journal Of Immunology. 28(2): 459-467,., 1998

Efficiency of genomic selection using Bayesian multi-marker models for traits selected to reflect a wide range of heritabilities and frequencies of detected quantitative traits loci in mice. Bmc Genetics 13: 42-42, 2013

Biophysical analysis of specific genomic loci assembled as chromatin in vivo. Methods in Enzymology 376: 17-29, 2004

DNA methylation analysis of human imprinted Loci by bisulfite genomic sequencing. Csh Protocols 2008: Pdb.Prot5046-Pdb.Prot5046, 2008

Genomic analysis of the CSN2 and CSN3 loci in two Czech goat breeds. Animal Science Papers and Reports 23(1): 67-70, 2005