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Multi-modality, In Vivo Imaging of Signal Transduction Pathway Activity



Multi-modality, In Vivo Imaging of Signal Transduction Pathway Activity



FASEB Journal 18(4-5): Abst 19 1



Recent developments in imaging signal transduction pathway activity will provide a better understanding of disease (tumor) development and progression, as well as a better assessment of new molecular-targeted drugs. Monitoring the signaling activity of cells in homogenous tissue regions of living organisms can be performed by non-invasive molecular imaging, with the appropriate target-probe combination (direct imaging) or by the development of inducible reporter systems. We will focus on the application of reporter transgenes to image the endogenous regulation of transcription, translation and post-translational protein-protein interactions in specific signaling pathways, as developed by us and by others. The application of cis-acting enhancer elements, sensitive to p53, NFAT, HIF-1α, TGF-β pathways will be discussed. Examples of imaging post-transcriptional modification using mRNA stabilization/destabilization sequences, and imaging post-translational protein-protein interactions will be presented. Other systems have been developed to image the activation of proteolytic enzymes, such as caspases (for apoptosis) or metalloproteases (for metastases). The advantages and cost effectiveness of multi-modality (nuclear, optical and magnetic resonance) reporter imaging systems will be stressed.

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Accession: 035349366

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