EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Multi-stage continuous culture fermentation of glucose-xylose mixtures to fuel ethanol using genetically engineered Saccharomyces cerevisiae 424A



Multi-stage continuous culture fermentation of glucose-xylose mixtures to fuel ethanol using genetically engineered Saccharomyces cerevisiae 424A



Bioresource Technology 101(4): 1277-1284



Multi-stage continuous (chemostat) culture fermentation (MCCF) with variable fermentor volumes was carried out to study the utilization of glucose and xylose for ethanol production via mixed sugar fermentation (MSF). Variable fermentor volumes were used to enable enhanced sugar utilization, accounting for differences in glucose and xylose utilization rates. Saccharomyces cerevisiae 424A-LNH-ST was used for fermentation of glucose-xylose mixtures. The dilution rates employed for continuous fermentation were based on earlier batch kinetic studies of ethanol production and sugar utilization. With a feed containing approximately 30 g L-1 glucose and 15 g L-1 xylose, cell washout was observed at a dilution rate of 0.8 h-1. At dilution rates below 0.5 h-1, complete glucose utilization was observed. Xylose consumption in the first-stage 1 L reactor was only 37% at the lowest dilution rate studied, 0.05 h-1. At this same flow rate, xylose consumption rose to 69% after subsequently passing through 3 and 1 L reactors in series, primarily due to the longer residence time in the 3 L reactor (0.0167 h-1 dilution rate).

(PDF same-day service: $19.90)

Accession: 035349569

Download citation: RISBibTeXText

PMID: 19811910

DOI: 10.1016/j.biortech.2009.09.042



Related references

Effect of salts on the Co-fermentation of glucose and xylose by a genetically engineered strain of Saccharomyces cerevisiae. Biotechnology for Biofuels 6(1): 83-83, 2013

Establishment of L-arabinose fermentation in glucose/xylose co-fermenting recombinant Saccharomyces cerevisiae 424A(LNH-ST) by genetic engineering. Applied Microbiology and Biotechnology 87(5): 1803-1811, 2010

Effect of acetic acid and pH on the cofermentation of glucose and xylose to ethanol by a genetically engineered strain of Saccharomyces cerevisiae. Fems Yeast Research 10(4): 385-393, 2010

Successful design and development of genetically engineered Saccharomyces yeasts for effective cofermentation of glucose and xylose from cellulosic biomass to fuel ethanol. Advances in Biochemical Engineering/Biotechnology 65: 163-192, 1999

Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization. Microbial Cell Factories 9(): 16-16, 2010

Continuous alcoholic fermentation of glucose/xylose mixtures by co-immobilized Saccharomyces cerevisiae and Candida shehatae. Applied Microbiology and Biotechnology 50(3): 309-313, 1998

Repeated-batch fermentations of xylose and glucose-xylose mixtures using a respiration-deficient Saccharomyces cerevisiae engineered for xylose metabolism. Journal of Biotechnology 150(3): 404-407, 2011

Fermentation of xylose/glucose mixtures by metabolically engineered Saccharomyces cerevisiae strains expressing XYL1 and XYL2 from Pichia stipitis with and without overexpression of TAL1. Bioresource Technology 68(1): 79-87, 1999

A genetic overhaul of Saccharomyces cerevisiae 424A(LNH-ST) to improve xylose fermentation. Journal of Industrial Microbiology & Biotechnology 38(5): 617-626, 2011

Continuous fermentation of feed streams containing D-glucose and D-xylose in a two-stage process utilizing immobilized Saccharomyces cerevisiae and Pachysolen tannophilus. Biotechnology and Bioengineering 32(9): 1104-1112, 1988

Continuous co-fermentation of cellobiose and xylose by engineered Saccharomyces cerevisiae. Bioresource Technology 149: 525-531, 2014

Enhanced xylose fermentation and ethanol production by engineered Saccharomyces cerevisiae strain. Amb Express 5: 16-16, 2015

Modeling of the aerobic growth of Saccharomyces cerevisiae on mixtures of glucose and ethanol in continuous culture. Journal Of Biotechnology. 43(3): 213-220, 1995

Efficient fermentation of xylose to ethanol at high formic acid concentrations by metabolically engineered Saccharomyces cerevisiae. Applied Microbiology and Biotechnology 90(3): 997-1004, 2011