+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Multicenter comparison of real time PCR assays and an LCx PCR research test for detection and quantitation of Chlamydia pneumoniae DNA



Multicenter comparison of real time PCR assays and an LCx PCR research test for detection and quantitation of Chlamydia pneumoniae DNA



Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 43: 188



Background: A number of real time PCR assays have been developed for the detection and quantitation of Chlamydia pneumoniae DNA. Our objective was to compare the quantitative ability of Real Time PCR assays with a qualitative industry-derived assay, LCx(R) C. pneumoniae research use only (RUO; Abbott Laboratories). Methods: Serial 10-fold dilutions were made from nucleic acid extracted from 6 well characterized isolates and the end point was determined using a nested PCR. Samples of the end point and 3 preceding dilutions and 6 negatives were included in the panel (n=30). Two panels of these samples in different random orders were sent to 8 laboratories from North America and Europe, and the participants performed their respective assays on each of the panels on separate days. The LCx(R) RUO was performed at 1 site by a second blinded technologist. Results: Overall, real time PCR sensitivity was 377/384 (98.2%; 95% CI: 96.3, 99.1) and specificity was 93/96 (96.9%; 95% CI: 91.2, 98.9). The laboratories showed high quantitative intra-laboratory reproducibility (Spearman rho=0.97, P<0.001); however, the genomic copy number reported differed between sites by as much as 2.6 log10 units. The LCx(R) RUO had a sensitivity of 48/48 (100%; 95% CI: 92.6, 100) and a specificity of 12/12 (100%; 95% CI: 75.8, 100). Conclusions: Analytical sensitivity and specificity for the real time assays and the LCx(R) RUO were very high. Laboratories showed high quantitative intra-laboratory reproducibility; however, universal standards may be necessary for reporting absolute numbers.

(PDF emailed within 1 workday: $29.90)

Accession: 035349753

Download citation: RISBibTeXText


Related references

Real-time PCR assays for the detection of Mycoplasma pneumoniae and Chlamydia pneumoniae on throat swabs. Journal of Allergy & Clinical Immunology 111(2 Abstract Supplement): S307, February, 2003

Comparison of a new quantitative ompA-based real-Time PCR TaqMan assay for detection of Chlamydia pneumoniae DNA in respiratory specimens with four conventional PCR assays. Journal of Clinical Microbiology 41(2): 592-600, 2003

Simultaneous detection of Chlamydia pneumoniae and Chlamydia trachomatis DNA by real-time PCR FT Soucasny prukaz DNA Chlamydia pneumoniae a Chlamydia trachomatis pomoci real-time PCR. 2007

Development and evaluation of real-time PCR-based fluorescence assays for detection of Chlamydia pneumoniae. Journal of Clinical Microbiology 40(2): 575-583, 2002

Multicenter comparison trial of DNA extraction methods and PCR assays for detection of Chlamydia pneumoniae in endarterectomy specimens. Journal of Clinical Microbiology 39(2): 519-524, 2001

The pneumoplex assays, a multiplex PCR-enzyme hybridization assay that allows simultaneous detection of five organisms, Mycoplasma pneumoniae, Chlamydia (Chlamydophila) pneumoniae, Legionella pneumophila, Legionella micdadei, and Bordetella pertussis, and its real-time counterpart. Journal of Clinical Microbiology 43(2): 565-571, 2005

Comparison of three in-house multiplex PCR assays for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis using real-time and conventional detection methodologies. Pathology 37(5): 364-370, 2005

Comparison of commercial and in-house real-time PCR assays used for detection of Mycoplasma pneumoniae. Journal of Clinical Microbiology 47(2): 441-444, 2009

Multicenter comparison of different real-time PCR assays for quantitative detection of Epstein-Barr virus. Journal of Clinical Microbiology 46(1): 157-163, 2007

Comparison of three real-time PCR assays for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in young pregnant women. Diagnostic Microbiology and Infectious Disease 83(4): 335-337, 2016

A simultaneous detection of Chlamydia pneumoniae and Chlamydia trachomatis DNA by real-time PCR. Epidemiologie, Mikrobiologie, Imunologie 56(4): 166-173, 2007

Determination of PCR efficiency in chelex-100 purified clinical samples and comparison of real-time quantitative PCR and conventional PCR for detection of Chlamydia pneumoniae. BMC Microbiology 2(17 Cited August 6, ), July 9, 2002

Comparison of a real time PCR assay with a nested PCR assay for detection of Chlamydia pneumoniae. Abstracts of the General Meeting of the American Society for Microbiology 103: C-181, 2003

Detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella spp. in clinical specimens using a single-tube multiplex real-time PCR assay. Diagnostic Microbiology and Infectious Disease 70(1): 1-9, 2011