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Multicenter comparison of the MicroScan dried MICroSTREP plus panel to a NCCLS reference panel for MIC testing of Streptococcus spp



Multicenter comparison of the MicroScan dried MICroSTREP plus panel to a NCCLS reference panel for MIC testing of Streptococcus spp



Abstracts of the General Meeting of the American Society for Microbiology 103: C-072



A multicenter evaluation was conducted to assess the accuracy and reliability of the MicroScan dried overnight MICroSTREP plus (MSP) MIC panel as compared to a frozen NCCLS Reference (REF) broth microdilution method for antimicrobial susceptibility testing of Streptococcus spp. Eighteen antimicrobials were evaluated at three sites and the study included three phases: Efficacy, Challenge and Reproducibility A total of 493 clinical isolates (243 S. pneumoniae, 84 viridans group and 166 beta-hemolytic streptococci), comprised of 232 fresh and 261 stock isolates were tested during the Efficacy phase. Essential agreement between the MSP and REF panels was greater than 91% and categorical agreement was greater than 93% for all antimicrobials. The major error rate was less than 3% for all antimicrobials. The very major error (VMJ) rate was less than or equal to 1.5% for 15/18 antimicrobials. VMJ error rates greater than 1.5% were observed with erythromycin (1.6%, 1/67 S. pneumoniae and 1/39 viridans group streptococci), clindamycin (2.4%, 1/26 S. pneumoniae) and chloramphenicol (11.8%, 2/17 S. pneumoniae). Essential agreement for chloramphenicol, which has no intermediate breakpoint, was 100%. During the Challenge phase an additional 53 S. pneumoniae and 17 non-pneumococcal Streptococcus spp. were evaluated at one site. Expected results for each of the challenge isolates were pre-determined. For all 18 antimicrobials, the essential agreement was greater than 95% and categorical agreement was greater than 92%. During the Reproducibility phase 25 strains were tested at all three sites. The interlaboratory reproducibility was greater than or equal to 96% for each of 18 antimicrobials tested. The new MicroScan MICroSTREP plus panel for antimicrobial susceptibility testing of S. pneumoniae and other Streptococcus spp. shows excellent correlation in comparison to a frozen reference broth microdilution method. The room temperature storage and option for manual reading makes MICroSTREP plus attractive for laboratories that wish to perform MIC tests on Streptococcus spp. using a panel containing a wide variety of antimicrobial agents.

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