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Multicenter evaluation of a Candida albicans PNA FISH probe in blood cultures that contained yeast



Multicenter evaluation of a Candida albicans PNA FISH probe in blood cultures that contained yeast



Abstracts of the General Meeting of the American Society for Microbiology 103: C-162



Background: Fluorescent In-Situ Hybridization (FISH) is established as a diagnostic tool in some areas of laboratory medicine and is now being applied in microbiology. A new FISH procedure using a peptide nucleic acid (PNA) probe specific for Candida albicans 16S rRNA has been developed; the performance of this probe was evaluated in a multicenter study. Materials and Methods: Blood cultures systems used were the BD BactecTM 9600, BD BactecTM 9240, and Organon Teknika BacT Alert systems. The media used were the BD Bactec(R): resin, standard (aerobic/anaerobic), and Lytic 10; as well as BacT/ALERT(R): FA, FN, SN, SA, PSN and BacT/ALERTTM PF (plastic; for investigational use only). An aliquot from positive blood culture bottles that contained yeast on Gram stain were tested with the C. albicans PNA FISH probe, according to the manufacturer's instructions, which takes approximately 2.5 hours. Culture was used as the comparative standard, with the API 20C system used as the definitive identification system for any discrepant results. Results: Two hundred seventeen blood culture bottles from 201 patients that demonstrated yeast on Gram stain were evaluated by C. albicans PNA FISH. 94/217 cultures were initially reported culture-positive for C. albicans, but only 90 were positive by the C. albicans PNA FISH test. The re-evaluation of the four discrepant isolates with the API 20C system identified two as C. dubliniensis, one as C. parapsilosis and one as C. albicans. Three blood culture bottles that contained C. albicans, also contained a second yeast species; the non-C. albicans yeast forms, however, were not clearly visible in the PNA FISH preparation. The 126 cultures that grew a yeast other than C. albicans were negative by C. albicans PNA probe. The sensitivity and specificity of the C. albicans PNA FISH assay was 98.9% and 100%, respectively. Discussion: The Candida albicans PNA FISH assay is a sensitive, specific, and rapid method of differentiating Candida albicans from other yeast in samples taken directly from a variety of blood culture bottles that contain yeast. Additional probes would be necessary to detect other yeast.

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Accession: 035349773

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