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Multicenter evaluation of cation-adjusted Mueller-Hinton broth for antimicrobial susceptibility testing of aminoglycosides on MicroScan Dried Overnight Gram-Negative panels



Multicenter evaluation of cation-adjusted Mueller-Hinton broth for antimicrobial susceptibility testing of aminoglycosides on MicroScan Dried Overnight Gram-Negative panels



Abstracts of the General Meeting of the American Society for Microbiology 102: 121



The NCCLS recommends the use of cation-adjusted Mueller-Hinton broth (CAMHB) for antimicrobial susceptibility testing of aminoglycosides. MicroScan Dried Overnight (Conventional) Gram-Negative (GN) panels were reformulated to contain aminoglycosides in CAMHB. A multicenter evaluation was conducted to verify the accuracy and reliability of using this broth for testing of amikacin, gentamicin, tobramycin and netilmicin on MicroScan Conventional GN panels. The clinical study was conducted at four sites and consisted of two phases: efficacy and QC reproducibility. A total of 216 gram-negative clinical isolates (141 P. aeruginosa and 75 other) were tested on the GN panel during the efficacy phase with results compared to an NCCLS frozen reference panel. The QC reproducibility study evaluated the NCCLS recommended QC strains, Escherichia coli ATCC 25922 and P. aeruginosa ATCC 27853, prepared using the turbidity method of inoculation and tested with the WalkAway System, the autoSCAN-4 and manual reads as compared to the expected range. Essential agreements during the efficacy phase were greater than 98% and categorical agreements ranged from 96.2% to 98.1% for all four antimicrobics as compared to the NCCLS reference panel. QC reproducibility results for E. coli ranged from 97.2% to 100% and for P. aeruginosa from 99.4% to 100% for all read methods. The results of this study confirm that using CAMHB for antimicrobial susceptibility of aminoglycosides on MicroScan Conventional Gram-Negative panels gave excellent correlation when compared with an NCCLS reference method.

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Accession: 035349796

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