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Multicenter evaluation of the BD Phoenix Automated Microbiology System for antimicrobial susceptibility testing of Streptococcus spp



Multicenter evaluation of the BD Phoenix Automated Microbiology System for antimicrobial susceptibility testing of Streptococcus spp



Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 43: 170



Background: Increasing antimicrobial resistance in Streptococus pneumoniae (SP) and other streptococci highlights the importance of accurate and convenient AST methods for these strains in clinical labs. This multicenter study evaluated a new BD PhoenixTM Automated Microbiology System STREP AST panel (BD Diagnostic Systems). Methods: AST with 32 agents was performed on 1676 streptococci (47% SP, 21% group B strep (GBS), 18% viridans group strep (VGS), 13% beta-hemolytic strep group ACG (ACG)) using the Phoenix and a broth microdilution reference method in parallel. Clinical isolates from 4 sites and 92 challenge isolates from BD were tested. Accuracy of the Phoenix was measured as categorical agreement (CA, Phoenix=reference S/I/R result); very major errors (VME, false susceptible), and major errors (ME, false resistance). Results: Amoxicillin/clavulanate (AMC), cefotaxime (CTX), chloramphenicol (CHL), clindamycin (CLI), erythromycin (ERY), levofloxacin (LVX), penicillin (PEN), quinupristin/dalfopristin (QD), tetracycline (TET), and vancomycin (VA) showed these trends: For SP, CA was 98-100% for CHL, CLI, ERY, LVX, QD, TET, and VA; 93-96% for AMC, CTX, and PEN. ME were 0-0.4% and VME were noted with AMC (4.2%), CTX (6.4%), ERY (2%), PEN (0.5%), and TET (1.2%). For GBS, CA was 98-100% for all drugs except CLI (91%). There was only 1 VME for GBS (TET, 0.3%), but increased ME for CLI (7%). For VGS, CA was 94-100% for all drugs except TET (90.3%) and PEN (88.6%). The only VME for VGS occurred with CTX (7.7%), ERY (5.6%), and TET (6%). ME for VGS were 0-2%. For ACG, CA was 96-100% with only one VME (ERY, 3%) and two ME (TET, 0.5%; CLI, 2.8%). The mean time to generate all AST results was 8.8 h. Conclusions: The Phoenix provided reliable and rapid AST results with the exception of a few streptococcal/drug combinations.

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Accession: 035349805

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