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Multicenter evaluation of the Osiris system for the identification of Pseudomonas aeruginosa beta-lactam susceptibility phenotypes



Multicenter evaluation of the Osiris system for the identification of Pseudomonas aeruginosa beta-lactam susceptibility phenotypes



Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 41: 156



Background: Osiris(R) (Bio-Rad) is a video zone reader for disk diffusion tests with a built-in Extended Expert System (EES). The objective was to evaluate the efficacy of the Osiris EES for identifying beta-lactam susceptibility phenotypes in Pseudomonas aeruginosa. Methods: 13 b-lactams were tested in four laboratories by the disk diffusion test against 53 P. aeruginosa strains (7 "wild-type" and 46 with genotypically characterized resistance mechanisms). Antibiograms were read and interpreted with the Osiris(R) system. Strains were screened for the presence of Extended - Spectrum Beta-Lactamases (ESBL) by a synergy test between ceftazidime and clavulanic acid with disks placed at 3, 2, 1.5 and 1 cm, respectively. Results: For each genotype, the overall percentage of strains with correct phenotype identification was given. The absence of phenotype identification or misidentifications were observed for 3 strains with low-level penicillinase, 2 strains with derepressed cephalosporinase and 1 strain with high-level active efflux. Only 4 ESBLs (33%) were detected by a synergy between disks placed 3 cm apart. Five further ESBLs were identified at 2 cm, 2 were detected at 1.5 cm and the last one was identified only in 2 centers at 1 cm. Conclusion: The Osiris(R) EES is effective for recognizing most P. aeruginosa susceptibility phenotypes. A synergy test with disks placed at 2 and 1 cm is necessary to detect ESBLs.

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Accession: 035349816

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