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Multicenter evaluation of the proposed EUCAST antifungal susceptibility testing method



Multicenter evaluation of the proposed EUCAST antifungal susceptibility testing method



Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 41: 372



Background: The Subcommittee of AFST of The European Committee on Antibiotic Susceptibility Testing has proposed a standard AFST for yeasts. This AFST is based on the reference NCCLS M27-A procedure, but incorporates modifications (JCM 39:525-32, 2001). We have evaluated its intra and inter-laboratory reproducibility. Methods: Nine laboratories participated in the study. Common material lots were used to test 6 Candida strains (1 each C. albicans, C. tropicalis, C. parapsilosis, C. glabrata, C. krusei, C. lusitaniae), and two QC strains (C. krusei ATCC6258 and C. parapsilosis ATCC22019). Triplicate testing on 3 separate days vs. flucytosine (5FC), fluconazole (FLZ), and itraconazole (ITZ) was performed in RPMI-2% glucose. Agreement (AGR, % of MICs within one 2X dilution of the mode) and intraclass correlation coefficient (ICC, maximum value of 1) are shown. Results: The intra-lab AGR was >97% and >94.5% after 24h and 48h of incubation, respectively, with ICCs of >0.98 and >0.97. Two strains exhibiting trailing effect showed AGR of <92% and ICCs of <0.90 at 48h. The inter-lab AGR among 7 labs was 93% and 87% after 24h and 48h, respectively with ICCs of 0.98 and 0.97. The lowest values of AGR and ICC were obtained for ITZ (<84% and <0.93 respectively). Conclusions: (1) The procedure EUCAST AFST is a reproducible within and between labs. (2) Strains with trailing growth show reduced agreement and ICCs at 48h.

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