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Nucleotide sequence analysis of the ribosomal 58S and the internal transcribed spacer regions 1 and 2 from North American Armillaria species



Nucleotide sequence analysis of the ribosomal 58S and the internal transcribed spacer regions 1 and 2 from North American Armillaria species



Phytopathology 93(6 Suppl.): S12



Armillaria species are responsible for significant losses of woody plants worldwide, including North America, where ten pathogenic species have been identified. Very little is known about the inter- and intra-species genetic diversity in these fungi. The internal transcribed spacer regions 1 and 2 (ITS1 and ITS2) and the 5.8S region of the 18S ribosomal DNA were PCR amplified from three isolates each of ten pathogenic Armillaria species. Amplification products were cloned, and the ITS1-5.8S-ITS2 region from six clones were sequenced. Our results indicate that most isolates were heterozygous. Surprisingly, some diploid isolates had more than two alleles, indicating that the mechanism of concerted evolution does not seem fully operational. Because concerted evolution occurs most frequently during sexual recombination events, our results indicate that such events are rare in Armillaria species. Implications of these findings will be discussed.

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