+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Overexpression of a gibberellin inactivation gene alters seed development, KNOX gene expression, and plant development in Arabidopsis



Overexpression of a gibberellin inactivation gene alters seed development, KNOX gene expression, and plant development in Arabidopsis



Physiologia Plantarum 138(1): 74-90



We have examined the role of gibberellins (GAs) in plant development by expression of the pea GA 2-oxidase2 (PsGA2ox2) cDNA, which encodes a GA inactivating enzyme, under the control of the MEDEA (MEA) promoter. Expression of MEA:PsGA2ox2 in Arabidopsis caused seed abortion, demonstrating that active GAs in the endosperm are essential for normal seed development. MEA:PsGA2ox2 plants had reduced ovule number per ovary and exhibited defects in phyllotaxy and leaf morphology which were partly suppressed by GA treatment. The leaf architecture and phyllotaxy defects of MEA:PsGA2ox2 plants were also restored by sly1-d which reduces DELLA protein stability to increase GA response. MEA:PsGA2ox2 seedlings had increased expression of the KNOTTED1-like homeobox (KNOX) genes, BP, KNAT2 and KNAT6, which are known to control plant architecture. The expression of KNOX genes is also altered in wild-type plants treated with GA. These results support the conclusion that GAs can suppress the effects of elevated KNOX gene expression, and raise the possibility that localized changes in GA levels caused by PsGA2ox2 alter the expression of KNOX genes to modify plant architecture.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 035444884

Download citation: RISBibTeXText

PMID: 19825007

DOI: 10.1111/j.1399-3054.2009.01289.x


Related references

Overexpression of the KNOX gene Tkn4 affects pollen development and confers sensitivity to gibberellin and auxin in tomato. Plant Science 281: 61-71, 2019

Overexpression of a Knotted-like Homeobox Gene of Potato Alters Vegetative Development by Decreasing Gibberellin Accumulation. Plant Physiology 132(1): 106-117, 2003

Overexpression of a knotted-like homeobox gene of potato alters vegetative development by decreasing gibberellin accumulation. Plant Physiology 132(1): 106-117, 2003

Over-expression of an arabidopsis family A sucrose phosphate synthase (SPS) gene alters plant growth and fibre development. Transgenic Research 17(2): 181-192, 2008

Ectopic expression of pumpkin gibberellin oxidases alters gibberellin biosynthesis and development of transgenic Arabidopsis plants. Plant Physiology 140(2): 528-536, 2006

Ectopic Expression of Pumpkin Gibberellin Oxidases Alters Gibberellin Biosynthesis and Development of Transgenic Arabidopsis Plants. Plant Physiology 140(2): 528-536, 2006

Caged gene-inducer spatially and temporally controls gene expression and plant development in transgenic Arabidopsis plant. Bioorganic and Medicinal Chemistry Letters 16(9): 2470-2474, 2006

Modification of gibberellin production and plant development in Arabidopsis by sense and antisense expression of gibberellin 20-oxidase genes. Plant Journal 17(5): 547-556, 1999

Overexpression of RcLEC1-B, a HAP3 transcription factor of PLB from Rosa canina, increases the level of endogenous gibberellin and alters the development of cuticle and floral organs in Arabidopsis. Gene 688: 119-131, 2019

Overexpression of the Brassica napus BnLAS gene in Arabidopsis affects plant development and increases drought tolerance. Plant Cell Reports 30(3): 373-388, 2011

The Arabidopsis MCM2 gene is essential to embryo development and its over-expression alters root meristem function. New Phytologist 184(2): 311-322, 2009

The Arabidopsis Mcm2 Gene Is Essential to Embryo Development and Its Over-Expression Alters Root Meristem Function. New Phytologist 184(2): 311-322, 2009

The Arabidopsis DELAY OF GERMINATION 1 gene affects ABSCISIC ACID INSENSITIVE 5 (ABI5) expression and genetically interacts with ABI3 during Arabidopsis seed development. Plant Journal 85(4): 451-465, 2016

The KNOX gene SHOOT MERISTEMLESS is required for the development of reproductive meristematic tissues in Arabidopsis. Plant Journal 50(5): 767-781, 2007

Analysis of gene expression patterns during seed coat development in Arabidopsis. Molecular Plant 4(6): 1074-1091, 2011