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Potyvirus Y necrotic strain in potato tuber-seed from Canada



Potyvirus Y necrotic strain in potato tuber-seed from Canada



Revista Mexicana de Fitopatologia 20(2): 206-209



Canada is the main supplier of potato seed tubers for Mexico. This commodity represents a high risk of introducing the necrotic strain of potato virus Y (PVYN), classified as A1 quarantine pest for Mexico. The specific and reliable detection of this virus strain requires serology and complimentary bioassays. In this work, such protocols are described on potato tubers introduced from Canada from 1997 to 1999. Enzimatic serology (ELISA) with four antisera was implemented, followed by mechanic inoculation to Nicotiana tabacum cv Samsun with positive samples. In 1997, sixteen samples from 172 shipments were positive for PVY, and seven out of those 16 were identified as the necrotic strain; in 1998, two out of 23 positive were identified as PVYN, and none from 99 in 1999 (14, 8.7, and 0%, respectively). The polyclonal antiserum 4C3 and the monoclonal O+C detected the virus, but monoclonals 1F5 and Rose specifically identified the presence of PVYN; this was confirmed by the bioassay using tobacco. It is concluded that detection of such virus strain should be carried out using antisera 4C3 and/or Rose; in addition, Canadian shipments improved their phytosanitary quality after implementation of the protocols described.

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