+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Potyvirus Y necrotic strain in potato tuber-seed from Canada

Potyvirus Y necrotic strain in potato tuber-seed from Canada

Revista Mexicana de Fitopatologia 20(2): 206-209

Canada is the main supplier of potato seed tubers for Mexico. This commodity represents a high risk of introducing the necrotic strain of potato virus Y (PVYN), classified as A1 quarantine pest for Mexico. The specific and reliable detection of this virus strain requires serology and complimentary bioassays. In this work, such protocols are described on potato tubers introduced from Canada from 1997 to 1999. Enzimatic serology (ELISA) with four antisera was implemented, followed by mechanic inoculation to Nicotiana tabacum cv Samsun with positive samples. In 1997, sixteen samples from 172 shipments were positive for PVY, and seven out of those 16 were identified as the necrotic strain; in 1998, two out of 23 positive were identified as PVYN, and none from 99 in 1999 (14, 8.7, and 0%, respectively). The polyclonal antiserum 4C3 and the monoclonal O+C detected the virus, but monoclonals 1F5 and Rose specifically identified the presence of PVYN; this was confirmed by the bioassay using tobacco. It is concluded that detection of such virus strain should be carried out using antisera 4C3 and/or Rose; in addition, Canadian shipments improved their phytosanitary quality after implementation of the protocols described.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 035530680

Download citation: RISBibTeXText

Related references

Control of Stewart's Wilt in Sweet Corn with Seed Treatment Insecticides. Plant Disease 84(10): 1104-1108, 2000

The involvement of protein kinases and protein phosphatases in signal transduction during neurotransmitter-induced stimulation of root water-pumping activity. Russian Journal of Plant Physiology 54(4): 487-490, 2007

Behavior of phosphate rock in soils and its availability to plants. Dissertation Absts 21(3): 415, 1960

Potato disease caused by a bacillus like virus. Kartofel' i ovoshchi: 5 40-41, 1976

First Report of Xanthomonas cucurbitae Causing Bacterial Leaf Spot of Pumpkin on Réunion Island. Plant Disease 92(11): 1591, 2008

Occurrence of potato cyst nematode globodera rostochiensis in bata kundi area pakistan. Pakistan Journal of Nematology 7(1): 67, 1989

Reaction of wheat varieties to leaf rust, stripe rust and powdery mildew under field conditions. Plant Disease Research 12(2): 145-148, 1997

Evidence for limited exchange of avian influenza viruses between seaducks and dabbling ducks at Alaska Peninsula coastal lagoons. Archives of Virology 156(10): 1813-1821, 2011

Detection of the phenomenon of resistance in the field, and consequences. Sciences Veterinaires Medecine Comparee 98(3): 197-210, 1996

Identifying genes potentially important in plant-pathogen interactions of the root rot pathogen Phellinus sulphurascens and its host Douglas-fir. Canadian Journal of Plant Pathology-Revue Canadienne de Phytopathologie 31(1): 132-132, 2009

Reaction of certain wheat cultivars in different soil textures to root rots disease caused by Fusarium moniliforme, F. graminearum and/or Drechslera sorokiniana. Assiut Journal of Agricultural Sciences 31(3): 213-226, 2000

Biological response of rat fed diets with high tuber content of conventionally bred and transgenic potato resistant to necrotic strain of potato virus (PVY(N)). I. Chemical composition of tubers and nutritional value of diets. Food control 16(8): 761-766, 2005

First Report of Erysiphe sedi on Sedum spectabile in North America. Plant Disease 85(11): 1207, 2001

Identification of an isoform of ornithine carbamoyltransferase that can effectively utilize canaline as a substrate from the leaves of Canavalia lineata. Plant Science Limerick 151(2): 145-151, 2000

Potato disease caused by combined infection with potato spindle tuber viroid and potato virus Y necrotic strain. Russian Agricultural Sciences 33(3): 162-165, 2007