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Probe Wavelength for Pulse CO-Oximetry

Probe Wavelength for Pulse CO-Oximetry

Anesthesiology Abstracts of Scientific Papers Annual Meeting ( ): Abstract No A-557

Introduction:Carbon monoxide (CO) can develop in anesthetic circuits leading to CO poisoning. Carboxyhemoglobin (COHb) cannot be detected by standard pulse-oximeters as they interpret its absorption at 660/940 nm as oxyhemoglobin (O2Hb). Standard methods of measuring (COHb) require a blood sample. After a surveying the absorption spectra of COHb, we hypothesized that COHb can be best quantified from the absorption of light at 630 and 730 nm. Methods: Three New Zealand white rabbits were anesthetized and breathed spontaneously via a tracheostomy. A pulse oximeter probe with LEDs emitting at 630/730 nm was placed on the rabbit's paw. The rabbits were exposed to varying levels of CO until (COHb) reached apprx40%. The raw signal was processed to calculate absorbance. We did not measure pH and blood gases. Results: In all three experiments, (COHb) was linearly related to the absorption of 630/730 wavelengths with the average relationship of ROA = (COHb)*1.03+0.83 where ROA is the ratio of absorbance of 630/730. Discussion:The rabbits showed a good correlation between (COHb) and absorbance at 630/730 nm, however, the relation was different from rabbit to rabbit and seemed to change over time. Possible factors affecting the relationship between (COHb) and absorbance at the 630/730 wavelengths include PCO2, and pH. Further investigations are warranted.

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Accession: 035556306

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