Section 36
Chapter 35,561

Process for the purification of human interleukin-1 receptor antagonist from recombinant E coli

Zanette, D.; Sarubbi, E.G.; Soffientini, A.

Official Gazette of the United States Patent and Trademark Office Patents 1271(1)


ISSN/ISBN: 0098-1133
Accession: 035560759

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A process for purifying human Interleukin-1 receptor antagonist (IL-1ra), obtained by fermenting a strain of recombinant E. coli, which comprises: a) loading the mixture to be purified, which has been buffered at a pH value lower than 6.2 and optionally diluted to reduce its ionic strength at a low value, onto a cationic exchange matrix and eluting said matrix with an aqueous buffered solution having a pH value from about 7.5 to 9.0 and a low ionic strength; and b) applying the eluate from the cationic exchange step, containing the desired IL-1ra protein, directly onto an anionic exchange matrix and eluting with an aqueous buffered solution having a pH value within the above range 7.5 to 9.0 and an increased ionic strength.

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