+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Proteomic profiling of cerebrospinal fluid to identify diagnostic biomarkers for amyotrophic lateral sclerosis

Proteomic profiling of cerebrospinal fluid to identify diagnostic biomarkers for amyotrophic lateral sclerosis

FASEB Journal 18(4-5): Abst 151 5

Amyotrophic lateral sclerosis (ALS) is a common adult motor neuron disease that chronically and progressively affects motor neurons in the post-central gyrus, brain stem and spinal cord. Recent advances in understanding ALS pathogenesis have not yielded diagnostic biomarkers or any long-term drug interventions. Proteomic technologies are a sensitive and valuable tool to discover biomarkers and novel therapeutic targets. We examined the proteomic profile of cerebrospinal fluid (CSF) from ALS patients and control subjects to identify potential disease specific protein biomarkers. Since the CSF is in intimate contact with motor neurons and glia affected during ALS, this medium likely contains the highest level of protein biomarkers. CSF samples were fractionated and profiled on Ciphergen protein chip arrays using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). We found statistically significant differences in approximately 13% of the protein peak intensities between control and ALS subjects. Furthermore, multivariate analysis using two different computer algorithms identified a series of protein peaks that predicted ALS versus control with high sensitivity and specificity. These protein peaks represent the first biomarker panel for this neurodegenerative disease. Continued investigations will yield the protein identity of each biomarker and the function of each protein in disease pathogenesis.

(PDF emailed within 1 workday: $29.90)

Accession: 035581930

Download citation: RISBibTeXText

Related references

Proteomic profiling of cerebrospinal fluid identifies biomarkers for amyotrophic lateral sclerosis. Journal of Neurochemistry 95(5): 1461-1471, 2005

Proteomic studies in the discovery of cerebrospinal fluid biomarkers for amyotrophic lateral sclerosis. Expert Review of Proteomics 14(9): 769-777, 2017

Label-Free LC-MS/MS Proteomic Analysis of Cerebrospinal Fluid Identifies Protein/Pathway Alterations and Candidate Biomarkers for Amyotrophic Lateral Sclerosis. Journal of Proteome Research 14(11): 4486-4501, 2016

Proteomic analysis of cerebrospinal fluid in amyotrophic lateral sclerosis. Experimental and Therapeutic Medicine 11(6): 2095-2106, 2016

Quantitative proteomic profiling of cerebrospinal fluid to identify candidate biomarkers for Alzheimer's disease. Proteomics. Clinical Applications 2018: E1800105-E1800105, 2018

Cerebrospinal fluid macrophage biomarkers in amyotrophic lateral sclerosis. Annals of Neurology 83(2): 258-268, 2018

Cerebrospinal Fluid Biomarkers for Kii Amyotrophic Lateral Sclerosis/Parkinsonism-Dementia Complex. Journal of Neurodegenerative Diseases 2013: 679089, 2013

Identification of miRNAs as Potential Biomarkers in Cerebrospinal Fluid from Amyotrophic Lateral Sclerosis Patients. Neuromolecular Medicine 18(4): 551-560, 2016

Proteomic analysis of the cerebrospinal fluid from patients with amyotrophic lateral sclerosis based on tandem mass spectrometry technique. Nan Fang Yi Ke Da Xue Xue Bao 39(4): 428-436, 2019

Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Bmc Medical Genomics 12(1): 7-7, 2019

Cerebrospinal fluid biomarkers for Alzheimer's disease in patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis with the C9ORF72 repeat expansion. Dementia and Geriatric Cognitive Disorders 39(5-6): 287-293, 2015

Is cerebrospinal fluid obtained for diagnostic purpose a good material for biomarker studies in amyotrophic lateral sclerosis?. Biomarkers 19(7): 571-577, 2015

Cerebrospinal fluid (CSF) findings in amyotrophic lateral sclerosis. Journal of Neurology 231(2): 75-78, 1984

Cerebrospinal fluid erythropoietin (EPO) in amyotrophic lateral sclerosis. Neuroscience Letters 416(3): 257-260, 2007

Cerebrospinal fluid cytotoxicity in lateral amyotrophic sclerosis. Neurologia 25(6): 364-373, 2011