+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Quercetin protects against ethanol-induced oxidative damage in rat primary hepatocytes

Quercetin protects against ethanol-induced oxidative damage in rat primary hepatocytes

Toxicology in Vitro 24(2): 516-522

The pathogenesis and progression of alcoholic liver disease (ALD) are associated with free radical injury and oxidative stress, which could be partially attenuated by antioxidants and free radical scavengers. Quercetin, one of the most widely distributed flavonoids in plants, is a natural antioxidant. The hypothesis that quercetin could prevent the ethanol-induced oxidative damage in hepatocytes was investigated. The ethanol-intoxicated (100mM for 8h) rat primary hepatocytes were post-treated (2h), simultaneously treated or pre-treated (2h) with quercetin respectively, while the time-dependent (0.5-8h) and dose-dependent (25-200muM) quercetin pre-treatment were used in the present study. The parameters of lactate dehydrogenase (LDH), aspartate transaminase (AST), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were determined to address the alterations of cell damage and antioxidant state after quercetin intervention. The toxic insult of ethanol to hepatocytes was challenged by quercetin and these parameters almost returned to the level of control group when hepatocytes were pre-treated with quercetin at the dose of 50muM for 2-4h before ethanol exposure. In conclusion, quercetin pre-treatment provided protection against ethanol-induced oxidative stress in hepatocytes and may be used as a new natural drug for the prevention and/or treatment of ALD.

(PDF emailed within 0-6 h: $19.90)

Accession: 035598814

Download citation: RISBibTeXText

PMID: 19327393

DOI: 10.1016/j.tiv.2009.03.006

Related references

Protection of quercetin against ethanol-induced oxidative damage in rat primary hepatocytes. Acta Nutrimenta Sinica 29(3): 288-291, 2007

Quercetin protects rat hepatocytes from oxidative damage induced by ethanol and iron by maintaining intercellular liable iron pool. Human and Experimental Toxicology 33(5): 534-541, 2014

Heme oxygenase-1 mediates the protective role of quercetin against ethanol-induced rat hepatocytes oxidative damage. Toxicology in Vitro 26(1): 74-80, 2012

N-acetylcysteine protects against fluoride-induced oxidative damage in primary rat hepatocytes. Toxicology in Vitro 27(8): 2279-2282, 2014

Hibiscus protocatechuic acid protects against oxidative damage induced by tert-butylhydroperoxide in rat primary hepatocytes. Chemico-Biological Interactions. 101(2): 137-148, 1996

Quercetin protects human hepatocytes from ethanol-derived oxidative stress by inducing heme oxygenase-1 via the MAPK/Nrf2 pathways. Journal of Hepatology 47(2): 253-261, 2007

The right choice of antihypertensives protects primary human hepatocytes from ethanol- and recombinant human TGF-β1-induced cellular damage. Hepatic Medicine 5: 31-41, 2013

Crocetin protects against oxidative damage in rat primary hepatocytes. Cancer Letters. 97(1): 61-67, 1995

Quercetin and its metabolites protect hepatocytes against ethanol-induced oxidative stress by activation of Nrf2 and AP-1. Food Science and Biotechnology 27(3): 809-817, 2018

Nuclear factor-kappaB protects cultured rat hepatocytes from ethanol-induced apoptosis Critical roles of oxidative stress and acetaldehyde during ethanol metabolism. Gastroenterology 118(4 Suppl 2 Part 1): AASLD A924, 2000

Quercetin, a flavonoid antioxidant, prevents and protects against ethanol-induced oxidative stress in mouse liver. Biological & Pharmaceutical Bulletin 26(10): 1398-1402, 2003

Quercetin protects hamster spermatogenic cells from oxidative damage induced by diethylstilboestrol. Andrologia 42(5): 285-290, 2011

Quercetin protects spermatogonial cells from 2,4-d-induced oxidative damage in embryonic chickens. Journal of Reproduction and Development 53(4): 749-754, 2007

Borneol administration protects primary rat hepatocytes against exogenous oxidative DNA damage. Mutagenesis 27(5): 581-588, 2013

Quercetin protects the rat kidney against oxidative stress-mediated DNA damage and apoptosis induced by lead. Environmental Toxicology and Pharmacology 30(3): 264-271, 2011