EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Reorganization of cytoskeleton and lung surfactant secretion in alveolar type II cells


Reorganization of cytoskeleton and lung surfactant secretion in alveolar type II cells



FASEB Journal 15(4): A495



ISSN/ISBN: 0892-6638

Secretion of lung surfactant requires the movement of lamellar bodies to the plasma membrane through cytoskeletal barrier at the cell cortex. We hypothesized that the cortical cytoskeleton undergoes a transient disassembly/reassembly in the stimulated type II cells. therefore allowing lamellar bodies access to the plasma membrane. Stabilization of cytoskeleton with Jasplakinolinde, a novel cell permeable actin microfilament stabilizer, caused a dose-dependent inhibition of lung surfactant secretion. Stimulation of A549 cells, a lung epithelium-derived cell, with terbutaline exhibited a transient disassembly of the F-actin as determined by the DNase I inhibition assay and staining with Oregon Green 488 Phalloidin. A similar result was also observed in primary culture of lung type II cells. Western blot analysis using anti-actin and anti-annexin II antibodies showed a transient increase of G-actin and annexin II in the soluble fraction of terbutaline-stimulated type II cells. Phorborl 12-myristate 13-acetate, an activator of protein kinase C, also decreased cortical fluorescence staining by phalloidin in type II cells. Treatment of type II cells with N-ethylmaleimide (NEM), a sulfhydryl reagent, resulted in a disruption of the cortical actin. The results suggest that cytoskeleton undergoes reorganization in the stimulated type II cells and annexin II tetramer plays a role in this process.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 035649213

Download citation: RISBibTeXText

Related references

Reorganization of cytoskeleton during surfactant secretion in lung type II cells: a role of annexin II. Cellular Signalling 16(1): 63-70, 2004

Micro-RNA-375 inhibits lung surfactant secretion by altering cytoskeleton reorganization. Iubmb Life 62(1): 78-83, 2010

Stimulation of lung surfactant secretion by endothelin-1 from rat alveolar type II cells. American Journal of Physiology 266(3 Pt 1): L255-L262, 1994

Knockdown of flotillin-2 inhibits lung surfactant secretion by alveolar type II cells. Cell Research 18(6): 701-703, 2008

A role of annexin II in lung surfactant secretion from alveolar epithelial type II cells. Journal of General Physiology 110(1): 28A-29A, 1997

Antibody against SP-A receptor increases surfactant secretion by cultured alveolar type II cells Insights into the regulation of surfactant secretion, and its inhibition by SP-A. Biology of the Neonate 67(Suppl 1): 88-89, 1995

Mechanical stretch activates piezo1 in caveolae of alveolar type I cells to trigger ATP release and paracrine stimulation of surfactant secretion from alveolar type II cells. FASEB Journal 34(9): 12785-12804, 2020

Inhibition of lung surfactant secretion from alveolar type II cells and annexin II tetramer-mediated membrane fusion by phenothiazines. Archives of Biochemistry and Biophysics 342(2): 322-328, 1997

Nitric oxide donor, spermine nonoate, modulates lung surfactant secretion from cultured alveolar type II cells. FASEB Journal 12(4): A491, 1998

Characterization of alpha-soluble N-ethylmaleimide-sensitive fusion attachment protein in alveolar type II cells: implications in lung surfactant secretion. American Journal of Respiratory Cell and Molecular Biology 29(3 Pt 1): 273-282, 2003

Beta-adrenergic-induced surfactant synthesis, secretion, and reutilization in fetal rabbit lung and isolated differentiating type II alveolar cells. American Journal of Obstetrics and Gynecology 158(2): 373-379, 1988

A surfactant protein-A receptor controls surfactant secretion by type II alveolar cells. FASEB Journal 8(4-5): A141, 1994

Surfactant apoproteins inhibit secretion of surfactant by rat alveolar type ii cells in culture. American Review of Respiratory Disease 133(4 Suppl): A118, 1986

Pulmonary surfactant apoprotein A structure and modulation of surfactant secretion by rat alveolar type II cells. Journal of Biological Chemistry 263(7): 3388-3394, 1988

Surfactant protein a sp a lipid interactions and regulation of surfactant secretion by alveolar type ii cells. FASEB Journal 4(7): A2149, 1990