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SHV-39 A new extended-spectrum beta-lactamase found throughout Chile



SHV-39 A new extended-spectrum beta-lactamase found throughout Chile



Abstracts of the Interscience Conference on Antimicrobial Agents and Chemotherapy 42: 116



Background: Klebsiella pneumoniae is a widespread nosocomial pathogen within Chilean hospitals. It has largely been treated with ceftriaxone and cefotaxime and resistance has increased markedly. Methods: K. pneumoniae strains were isolated from hospitalised patients from the Catholic University hospital in Santiago, the Gustavo Fricke hospital in Valparaiso (100 miles to the east) and the G. Grant-Benavente hospital in Concepcion (300 miles to the south). The minimum inhibitory concentration of antibiotics was determined by agar dilution (NCCLS) and all strains were genotyped by pulsed-field gel electrophoresis (PFGE). The number of beta-lactamases was determined by iso-electric focusing. Results: Although there were some variations in genotype, within each hospital there was consistency of banding pattern (PFGE). However, there was no similarity between strains isolated in the different cities showing they were different clones. Examination of the beta-lactamases showed that TEM-1 was always present; however the cephalosporin resistance was associated with an SHV-derived beta-lactamase with a pI greater than 7.0. PCR amplification and sequencing revealed a hitherto undescribed enzyme with not just a Gly238Ser mutation, characteristic of SHV-2, but also a Thr267Ser mutation. This extended-spectrum beta-lactamases (ESBL) conferred MICs of ceftazidime up to greater than 256 mg/L and of cefotaxime between 16 and 32 mg/L. Conclusions: These results show that there is a new ESBL, now called SHV-39 that is widely disseminated in Chile both geographically and between different strains of Klebsiella pneumoniae.

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