+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Sensitization profiles in birch pollen-allergic patients with and without oral allergy syndrome to apple: lessons from multiplexed component-resolved allergy diagnosis

Sensitization profiles in birch pollen-allergic patients with and without oral allergy syndrome to apple: lessons from multiplexed component-resolved allergy diagnosis

Clinical and Experimental Allergy 40(2): 339-347

Component-resolved diagnosis (CRD) using microarray technology has recently been introduced into the field of clinical allergology. To further validate the use of CRD by microarray technology in allergy diagnosis. Thiry-seven patients allergic to birch pollen were included. The discriminative value of apple-specific IgE (sIgE), recombinant Mal d 1 (rMal d 1) sIgE, apple skin prick test and rMal d 1 on the microarray was assessed between patients with a birch-related oral allergy syndrome to apple (OAS(+), n=20) and healthy control individuals (HC, n=8) without a history of inhalant allergies or apple-induced OAS. An additional comparative analysis was carried out with individuals allergic to birch pollen allergy without OAS (OAS(-); n=17). rMal d 1 coupled to the microarray constitutes a discriminative marker between OAS(+) and HC with a sensitivity 95% and a specificity of 100%. However, in parallel with the traditional sIgE assay, 15 out of 17 OAS(-) individuals (88%) also displayed IgE reactivity to rMal d 1 coupled to the microarray. OAS(-) individuals are more frequently sensitized to mite (about three to four times), cat and dog dander (about two to three times) and grass pollen (about 1.5 times) as compared with OAS(+) patients. At first glance, CRD by microarray seems to be a reliable instrument in the diagnosis of apple-mediated OAS in birch pollen allergy. However, for discriminating between sensitization and a real allergy, micro-arrayed rMal d 1 offers no advantage over conventional quantification of rMal d 1 sIgE. Most interestingly, within a single run, birch pollen-allergic patients without OAS to apple were shown to display a broader sensitization to classical inhalant allergens than birch pollen-allergic patients with an apple-related OAS.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 035711887

Download citation: RISBibTeXText

PMID: 19709127

DOI: 10.1111/j.1365-2222.2009.03345.x

Related references

High correlation of double Debye model parameters in skin cancer detection. Conference Proceedings 2014: 718-721, 2014

Clinical association of anti-glutathione S-transferase T1 antibodies and de novo immune hepatitis after hematopoietic cell transplantation. Digestive and Liver Disease 50(4): 418-419, 2018

Clinical benefit of atrio-ventricular delay optimization in patients with a dual-chamber pacemaker: a pilot study. Acta Cardiologica 71(3): 257-265, 2016

Art as Sanctuary: A Four-Year Mixed-Methods Evaluation of a Visual Art Course Addressing Uncertainty Through Reflection. Academic Medicine 93(11s Association of American Medical Colleges Learn Serve Lead: Proceedings of the 57th Annual Research in Medical Education Sessions): S8, 2018

Recombinant bacteriophage-based multiepitope vaccine against Taenia solium pig cysticercosis. Veterinary Immunology and Immunopathology 99(1-2): 11-24, 2004

Sensitization profile in differential diagnosis: allergic asthma vs. chronic (nonspecific) cough syndrome. Medical Science Monitor 19: 409-415, 2013

Validation of a astemizole binding assay in HEK293 cells expressing HERG K+ channels. Journal of Pharmacological Sciences 95(3): 311-319, 2004

Validation of a Measure of Ethical Sensitivity and Examination of the Effects of Previous Multicultural and Ethics Courses on Ethical Sensitivity. Ethics and Behavior 13(3): 221-235, 2003

Oral Administration of Methylphenidate Induces Hippocampal Necrosis in Juvenile Rats. Neurosurgery Quarterly 25(3): 411-414, 2015

Oral Allergy Syndrome Due to Nut Oleosins. Journal of Investigational Allergology and Clinical Immunology 25(4): 301-302, 2015

Clustering of food and activity preferences in primary school children. Appetite 60(1): 123-132, 2013

Allergy to antibiotics in children: an overestimated problem. International Journal of Antimicrobial Agents 48(4): 361-366, 2016

Sensitization to turnip rape and oilseed rape in children with atopic dermatitis: a case-control study. Pediatric Allergy and Immunology 19(5): 408-411, 2008

Profiles of African American College Students' Alcohol Use and Sexual Behaviors: Associations With Stress, Racial Discrimination, and Social Support. Journal of Sex Research (): 1-12, 2016

Basophil allergen threshold sensitivity, CD-sens, is a measure of allergen sensitivity in asthma. Clinical and Experimental Allergy 41(8): 1091-1097, 2011