+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Sensory receptive properties and conduction velocities of dorsal root ganglion neurones that express the PN1 Na+ channel alpha subunit



Sensory receptive properties and conduction velocities of dorsal root ganglion neurones that express the PN1 Na+ channel alpha subunit



Society for Neuroscience Abstracts 26(1-2): Abstract No. 352-20



Intracellular voltage recordings were made in L6 and S1 DRG neurones of deeply anaesthetised young guinea pigs. Intracellular action potentials were evoked by stimulation of the dorsal root, and the CV of the dorsal root fibre estimated from the latency. Units were identified as nociceptive or low threshold mechanoreceptive (LTM) by stimulation of the receptive field on the hind limb. Fluorescent dye was then injected into the neuronal soma. After fixation and sectioning, ABC immunocytochemistry was carried out on about 30 identified nociceptive and 30 identified non-nociceptive units using a site directed PN1 sodium channel antibody (ToledoAral et al., 1997). Although positive PN1-like immunoreactivity was seen in all C-fibre nociceptive units, most Adelta nociceptive units and less than half the Abeta nociceptive units, it was also seen in all Adelta low threshold mechanoreceptive units and in about half the cutaneous afferent Aalpha/beta units, but not in any muscle spindle afferent units. This pattern raised the question of whether PN1-like immunoreactivity in the soma was more related to fibre CV than to sensory receptive properties. This proved to be the case with PN1 intensity being inversely related to dorsal root fibre CV. The most intensely positive neurones had small cell bodies. In conclusion, the intensity of PNl-like immunoreactivity in primary afferent neurones was not related to sensory property but was related to CV and cell size, with the greatest intensity in small, slowly conducting primary afferent neurones.

Accession: 035712392

Download citation: RISBibTeXText


Related references

Electrical properties of rat dorsal root ganglion neurones with different peripheral nerve conduction velocities. Journal of Physiology 359: 47-63, 1985

Sensory receptor type and conduction velocity of A-fibre dorsal root ganglion neurones that express TrkA in the rat in vivo. European Journal of Neuroscience 12(Suppl. 11): 484, 2000

Association of somatic action potential shape with sensory receptive properties in guinea-pig dorsal root ganglion neurones. Journal of Physiology (Cambridge) 513(3): 857-872, 1998

Sensory receptor type and electrophysiological properties of A-fiber dorsal root ganglion neurones that express trkA in the rat. Society for Neuroscience Abstracts 25(1-2): 1946, 1999

Immunohistochemical demonstration of the calcium channel alpha 2 subunit in the chicken dorsal root ganglion and spinal cord: A special reference to colocalization with calbindin-D28k in dorsal root ganglion neurons. Neuroscience Research 59(3): 304-308, 2007

Intracellular recordings from dorsal root ganglion neurones in the anaesthetized guinea-pig to allow comparisons of afferent receptive and cytochemical properties. Journal of Physiology (Cambridge) 504P: 58P, 1997

Calcitonin gene-related peptide immunoreactivity and afferent receptive properties of dorsal root ganglion neurones in guinea-pigs. Journal of Physiology (Cambridge) 540(3): 989-1002, 2002

Sensory receptor properties of isolectin B4-positive and -negative dorsal root ganglion neurones in vivo in the rat. Society for Neuroscience Abstracts 26(1-2): Abstract No. 354-9, 2000

Streptozocin-induced neuropathy is associated with altered expression of voltage-gated calcium channel subunit mRNAs in rat dorsal root ganglion neurones. Biochemical and Biophysical Research Communications 289(2): 402-406, 2001

Sensory and electrophysiological properties of guinea-pig sensory neurones expressing Nav 1.7 (PN1) Na+ channel alpha subunit protein. Journal of Physiology 546(Pt 2): 565-576, 2003

Sensory properties of normal rat dorsal root ganglion neurons that express C - ret and changes in C - ret expression after nerve injury. Society for Neuroscience Abstract Viewer and Itinerary Planner : Abstract No 586 1, 2003

Correlation of CGRP-like immunoreactivity with sensory receptor properties in dorsal root ganglion neurones in guinea-pigs. Journal of Physiology (Cambridge) 493P: 45P, 1996

Voltage-dependent binding and calcium channel current inhibition by an anti-α1D subunit antibody in rat dorsal root ganglion neurones and guinea-pig myocytes. Journal of Physiology 502(2): 307-319, 1997

Voltage-dependent binding and calcium channel current inhibition by an anti-a1D subunit antibody in rat dorsal root ganglion neurones and guinea-pig myocytes. The Journal of Physiology 502: 7-19, 1997

Methodological considerations in studies of correlations between peptide content and sensory receptor properties in dorsal root ganglion neurones. Journal of Physiology (Cambridge) 491P: 16S, 1996