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Single-molecule spectroscopy for nucleic acid analysis: a new approach for disease detection and genomic analysis

Single-molecule spectroscopy for nucleic acid analysis: a new approach for disease detection and genomic analysis

Current Pharmaceutical Biotechnology 5(3): 271-278

Recently developed single-molecule spectroscopy (SMS) permits the analysis of fluorescent mixtures one molecule at a time. SMS methods provide the means to make rapid measurements on small, complex samples without the need for separations and target amplification enabling a new class of ultrasensitive nucleic acid assays. Here we give a brief overview of the current state of the art of SMS nucleic acid analysis and discuss ongoing work in our laboratory on two-color single-molecule fluorescence detection of specific nucleic acid sequences. In the future, two-color SMS nucleic acid assays will be used for a variety of applications including: gene expression analysis, disease detection and genomics.

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Accession: 035735432

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PMID: 15180548

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