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The use of a single-stage gradostat reactor to investigate the ability of rhizosphere associated bacteria to cycle lambda-cyhalothrin through the production of a redox gradient



The use of a single-stage gradostat reactor to investigate the ability of rhizosphere associated bacteria to cycle lambda-cyhalothrin through the production of a redox gradient



Abstracts of the General Meeting of the American Society for Microbiology 103: Q-239



We investigated the ability of rhizosphere bacterial consortia to degrade lambda-cyhalothrin. A single-stage continuous gravimetric flow gradostat was constructed and the reduction-oxidation potential was measured throughout the column over time during the 26-day enrichment period. The gradostat consisted of a chromaflex low-pressure chromatography column filled with mystic white quartz sand for the fixed phase. A liquid mineral salt medium was utilized incorporating a modified version of Hutner's minimal medium and "a field concentration" of 0.5 mg/L of a 5% lambda-cyhalothrin solution as the sole carbon source. Rhizosphere samples of Juncus effusus were collected from constructed wetlands at the Center for Water and Wetland Resources at the University of Mississippi Field Station. From a 10-1 dilution of rhizosphere solution, 5 ml was used to inoculate the column. Enrichment media was fed into the column at a rate of 1 ml/min from a fluorinated NalgeneETAPR; carboy. At 5 days the redox potential (mV) at ports 1,6 and 11 was 256, 240 and 244 and at 15 days it was -9.3, 1.6 and -1.6 and at 26 days itwas 1.8, 0.4 and 0.3. Additionally solid and liquid basal medium enriched with a 5% lambda-cyhalothrin solution were inoculated with a rhizosphere solution from Juncus effusus. After 72 hrs at 30 C bacterial colonies were present on the solid medium. The colonies were picked and streaked onto fresh enrichment plates; this was repeated three times. From liquid media 1 ml samples were analyzed using GC-MS and >75% of the lambdacyhalothrin had been degraded. These results demonstrate the potential for the bacteria associated with the rhizosphere of Juncus effusus to degrade and cycle lambda-cyhalothrin through their ability to grow in basal and liquid medium enriched with a single carbon source in the form of lambda-cyhalothrin. The redox gradient generated in continuous flow culture, indicates concomitant oxygen consumption, (electron acceptor), resulting from the oxidation of lambda-cyhalothrin to produce a redox gradient through the course of the column demonstrating the use of lambda-cyhalothrin as an electron donor for these bacteria.

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Accession: 035941477

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