+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Treatment of Experimental Acute Severe Anemia with Recombinant Human Erythropoietin



Treatment of Experimental Acute Severe Anemia with Recombinant Human Erythropoietin



Anesthesiology Abstracts of Scientific Papers Annual Meeting (2000): Abstract No 495



INTRODUCTIONS: Unpredictable significant blood loss is encountered in cases of severe trauma and/or major surgery. Clinically, the perioperative administration of recombinant human erythropoietin (rHuEPO) is still restricted to selective operations in which patients' Hb level is between 10 and 13 grams/dL. The benefits of rHuEPO therapy is unclear when stated after surgical and/or traumatic blood loss. The purpose of this study was to investigate the potential application and efficacy of rHuEPO after experimental severe acute anemia. METHODS: 50 Sprague-Dawley rats, weighing 350g, were used in the study. Blood was removed through a carotid catheter and replaced with 6% hetastarch. The Hb was reduced to 50% of baseline on the first day. The following day, blood depletion continued until Hb was reduced to 30% (4mg/dL) of baseline. Blood pressure and heart rate were maintained within normal range during the phlebotomy. The anemic rats were randomly divided into two groups: the control group without any treatment (dietary iron) and the rHuEPO group, in which rats received rHuEPO (300 U/kg/day) subcutaneously daily for 10 days postphlebotomy. Hb, hematocrit (Hct), and reticulocyte counts (Ret) as well as serum iron were measured from baseline to 28 days postphlebotomy. RESULTS: Hb in both groups showed a gradual increase after phlebotomy. The Hb in the rHuEPO group grew much faster than that in the control group between days 6 and 14 (p < 0.05). It took 12 days for Hb's in the rHuEPO group recovery to return to baseline compared with 14 days in the control group. The pattern of Hct parallels the change of reticulocytes and began to increase on day 3, peaked on day 7 (34.37 +- 4.33 % in the control group; 46.84 +- 4.34 % in the rHuEPO group), and returned to baseline levels (3.74 +- 2.56 %) on day 21 in both groups. The rHuEPO group experienced a greater rate of increase in the mean reticulocyte count starting before day 3 to day 7 (p < 0.05). The decrease of circulating iron after phlebotomy was induced in both groups, but much lower levels were reached in the rHuEPO-treated group (p < 0.05). CONCLUSIONS: Post hemorrhage administration of rHuEPO in experimental severe acute anemia leads to faster restoration of reticulocytosis and hemoglobin. Normal iron stores are unable to keep up with the enhanced response to rHuEPO. Iron supplement is needed to optimizes the erythropoiesis enhanced by rHuEPO. This study suggests the potential application of rHuEPO trauma in severe blood loss setting and could reduce the possibility of patients' exposure to allogeneic transfusion.

(PDF emailed within 1 workday: $29.90)

Accession: 035980406

Download citation: RISBibTeXText


Related references

Room F, 10/17/2000 2: 00 PM - 4: 00 PM (PS) Treatment of Experimental Acute Severe Anemia with Recombinant Human Erythropoietin. Anesthesiology 93(Supplement): A-495, 2000

Serum erythropoietin levels in patients with severe anemia secondary to inflammatory bowel disease and the use of recombinant human erythropoietin in patients with anemia refractory to treatment. Diseases of the Colon and Rectum 38(8): 896-897, 1995

Successful treatment of refractory severe aplastic anemia with recombinant human granulocyte-colony stimulating factor , recombinant human erythropoietin and cyclosporin A. Blood 82(10 SUPPL 1): 91A, 1993

Human recombinant erythropoietin for the treatment of severe anemia in children with cancer. Proceedings of the American Association for Cancer Research Annual Meeting 32: 191, 1991

Human recombinant erythropoietin in the treatment of the severe anemia following labor (a preliminary report). Akusherstvo i Ginekologiia 38(4): 13-15, 2000

Treatment of acute postoperative anemia with recombinant human erythropoietin. Journal of Trauma 29(8): 1134-8; Discussion 1138-9, 1989

Treatment of perioperative acute anemia by human recombinant erythropoietin in a Jehovah's witness. Annales Francaises d'Anesthesie et de Reanimation 16(3): 312-313, 1997

Treatment of post-traumatic acute anemia by recombinant human erythropoietin in Jehovah's Witnesses. Annales Francaises d'Anesthesie et de Reanimation 15(8): 1199-1202, 1996

Treatment of severe aplastic anemia with an immunosuppressive agent plus recombinant human granulocyte-macrophage colony-stimulating factor and erythropoietin. American Journal of Hematology 59(3): 185-191, 1998

Successful treatment of the anemia of rheumatoid arthritis with subcutaneously administered recombinant human erythropoietin: Slower response in patients with more severe inflammation. Scandinavian Journal of Rheumatology 22(4): 188-193, 1993

Human recombinant erythropoietin rhuepo treatment of severe anemia associated with progressive renal failure may delay the need to initiate regular dialytic therapy. Kidney International 37(1): 240, 1990

Recombinant human erythropoietin for severe anemia in pregnancies complicated by renal disease. American Journal of Obstetrics & Gynecology 170(1 PART 2): 329, 1994

An experimental study of recombinant human erythropoietin on the treatment of acute myocardial infarction in rats. Zhonghua Yi Xue Za Zhi 86(39): 2776-2780, 2007

Antibodies to recombinant human erythropoietin in systemic lupus erythematosus Correlation with severe anemia. Arthritis & Rheumatism 40(9 SUPPL ): S217, Sept, 1997

Pilot study comparing recombinant erythropoietin alone with erythropoietin plus recombinant granulocyte-macrophage colony-stimulating factor for treatment of the anemia of prematurity. Journal of Perinatology 14(2): 110-113, 1994