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Trend of blaVIM positive Pseudomonas spp and detection of blaVIM variant at a Korean hospital from 1995 to 2002



Trend of blaVIM positive Pseudomonas spp and detection of blaVIM variant at a Korean hospital from 1995 to 2002



Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 43: 153



Background: The frequency of imipenem resistance among P. aeruginosa isolates in Korea increased since 1995. Approximately 9% of the imipenem-resistant isolates were metallo-beta-lactamase (MBL)-producers, and all of these had blaVIM-2 alleles. However, the types have not been determined. The aim of this study was to determine the prevalence of MBL-producing isolates and the type of MBL in Pseudomonas spp. in Korea. Methods: Pseudomonas spp. was isolated from patients at a Korean tertiary-care hospital from 1995 to 2002. The modified Hodge and imipenem-EDTA double disk synergy test were used to screen MBL-producing isolates among imipenem-nonsusceptible Pseudomonas strains. MBL gene was detected by PCR using VIM primer (VIM-2: 5'-ATG TTC AAA CTT TTG AGT AAG-3', and 5'-CTA CTC AAC GAC TGA GCG-3'). Presence of blaVIM variant was determined by denaturing high-performance liquid chromatography (DHPLC) using reference strains with known blaVIM-1, blaVIM-2 and blaVIM-3 sequences. Results: The resistance rate of P. aeruginosa to imipenem increased from 15% in 1999 to 32% in 2002. Among the imipenem-nonsusceptible isolates tested during 2000-2002, MBL production was detected in 84 of 769 (10.9%). All MBL-producers had blaVIM-2 alleles. blaVIM-2 alleles-positive strains, which were isolated in 1995-2002 and tested by DHPLC, were 125 P. aeruginosa and 32 P. putida. Most of these strains had the same DHPLC profile to that of reference blaVIM-2 strain. Only minor differences in DHPLC profile were observed in ten strains. However, analyses using the amplicons showed the nucleotide sequences in all of the ten isolates were identical to that of blaVIM-2 in the reference strain. Conclusions: Imipenem-resistance rate of P. aeruginosa increased significantly and the proportion of MBL-producing isolates remained high in 2002 in Korea. DHPLC suggested the types of MBL in all isolates of Pseudomonas spp. were VIM-2.

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Accession: 035985071

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