+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Trend of blaVIM positive Pseudomonas spp and detection of blaVIM variant at a Korean hospital from 1995 to 2002

Trend of blaVIM positive Pseudomonas spp and detection of blaVIM variant at a Korean hospital from 1995 to 2002

Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 43: 153

Background: The frequency of imipenem resistance among P. aeruginosa isolates in Korea increased since 1995. Approximately 9% of the imipenem-resistant isolates were metallo-beta-lactamase (MBL)-producers, and all of these had blaVIM-2 alleles. However, the types have not been determined. The aim of this study was to determine the prevalence of MBL-producing isolates and the type of MBL in Pseudomonas spp. in Korea. Methods: Pseudomonas spp. was isolated from patients at a Korean tertiary-care hospital from 1995 to 2002. The modified Hodge and imipenem-EDTA double disk synergy test were used to screen MBL-producing isolates among imipenem-nonsusceptible Pseudomonas strains. MBL gene was detected by PCR using VIM primer (VIM-2: 5'-ATG TTC AAA CTT TTG AGT AAG-3', and 5'-CTA CTC AAC GAC TGA GCG-3'). Presence of blaVIM variant was determined by denaturing high-performance liquid chromatography (DHPLC) using reference strains with known blaVIM-1, blaVIM-2 and blaVIM-3 sequences. Results: The resistance rate of P. aeruginosa to imipenem increased from 15% in 1999 to 32% in 2002. Among the imipenem-nonsusceptible isolates tested during 2000-2002, MBL production was detected in 84 of 769 (10.9%). All MBL-producers had blaVIM-2 alleles. blaVIM-2 alleles-positive strains, which were isolated in 1995-2002 and tested by DHPLC, were 125 P. aeruginosa and 32 P. putida. Most of these strains had the same DHPLC profile to that of reference blaVIM-2 strain. Only minor differences in DHPLC profile were observed in ten strains. However, analyses using the amplicons showed the nucleotide sequences in all of the ten isolates were identical to that of blaVIM-2 in the reference strain. Conclusions: Imipenem-resistance rate of P. aeruginosa increased significantly and the proportion of MBL-producing isolates remained high in 2002 in Korea. DHPLC suggested the types of MBL in all isolates of Pseudomonas spp. were VIM-2.

(PDF emailed within 1 workday: $29.90)

Accession: 035985071

Download citation: RISBibTeXText

Related references

Novel Variant (blaVIM-4) of the Metallo- -Lactamase Gene blaVIM-1 in a Clinical Strain of Pseudomonas aeruginosa. Antimicrobial Agents and ChemoTherapy 46(12): 4026-4028, 2002

Emergence of Pseudomonas aeruginosa with class 1 integron carrying blaVIM-2 and blaVIM-4 in the University Clinical Hospital of Bialystok (northeastern Poland). Postepy Higieny i Medycyny Doswiadczalnej 71(0): 589-594, 2017

Hospital outbreak of multiple clones of Pseudomonas aeruginosa carrying the unrelated metallo-beta-lactamase gene variants blaVIM-2 and blaVIM-4. Journal of Antimicrobial ChemoTherapy 51(6): 1409-1414, 2003

blaVIM-2 Cassette-Containing Novel Integrons in Metallo- -Lactamase-Producing Pseudomonas aeruginosa and Pseudomonas putida Isolates Disseminated in a Korean Hospital. Antimicrobial Agents and ChemoTherapy 46(4): 1053-1058, 2002

blaVIM-2 and blaVIM-7 carbapenemase-producing Pseudomonas aeruginosa isolates detected in a tertiary care medical center in the United States: report from the MYSTIC program. Journal of Clinical Microbiology 45(2): 614-615, 2006

BlaVIM subscript -2(B and blaVIM subscript -7(B Carbapenemase-Producing Pseudomonas aeruginosa Isolates Detected in a Tertiary Care Medical Center in the United States: Report from the MYSTIC Program. Journal of clinical microbiology JCM 45(2): 614-615, 2007

Dissemination of the blaIMP-1 and blaVIM-2 metallo-beta-lactamase genes among genetically unrelated Pseudomonas aeruginosa isolates in a South Korean hospital. International Journal of Antimicrobial Agents 31(6): 586-588, 2008

Evaluation of Etest MBL for detection of blaIMP-1 and blaVIM-2 allele-positive clinical isolates of Pseudomonas spp. and Acinetobacter spp. Journal of Clinical Microbiology 43(2): 942-944, 2005

Detection of blaVIM-2 carbapenemase in Pseudomonas aeruginosa in Ireland. Journal of Antimicrobial ChemoTherapy 61(1): 219-220, 2007

Molecular structure of blaVIM-2-carrying class 1 integrons in gram-negative bacilli isolated at a Korean hospital. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 42: 68, 2002

Molecular epidemiology of outbreak-related pseudomonas aeruginosa strains carrying the novel variant blaVIM-17 metallo-beta-lactamase gene. Antimicrobial Agents and ChemoTherapy 53(4): 1325-1330, 2009

Molecular Characterization of Pseudomonas putida Group Isolates Carrying blaVIM-2 Disseminated in a University Hospital in Korea. Microbial Drug Resistance, 2018

Draft Genome Sequence of a Pseudomonas sp. Strain Carrying blaIMP-25 and blaVIM-2 Carbapenemase Genes from Hospital Sewage. Genome Announcements 4(5): -, 2016

A novel class 1 integron array carrying blaVIM-2 genes and a new insertion sequence in a Pseudomonas aeruginosa strain isolated from a Spanish hospital. Journal of Medical Microbiology 60(Pt 7): 1053-1054, 2011

Predominance of carbapenem-resistant Pseudomonas aeruginosa isolates carrying blaIMP and blaVIM metallo-β-lactamases in a major hospital in Costa Rica. Journal of Medical Microbiology 64(Pt 1): 37-43, 2015