+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

VEGF, VEGF isoforms and VEGFR2 in human skeletal muscle



VEGF, VEGF isoforms and VEGFR2 in human skeletal muscle



FASEB Journal 17(4-5): Abstract No 320 8



The VEGF isoforms and their receptors are prime regulators, with overlapping but specific roles, in controlling the neovascularization. In the present study the main aim was to explore at the mRNA level if exercise induces a concurrent increase in VEGF and VEGF receptors and whether exercise induces an increase in specific VEGF isoforms. Additionally, we wanted to study if there was an additive response following exercise performed in a condition of enhanced metabolic stress.Method: Nine healthy male subjects performed a 45'bout of one-legged exercise during reduced blood flow as well as under normal flow condition. Biopsies were taken in vastus lateralis before and 2h and 6h after exercise. mRNA levels were analyzed with real time PCR.Results: Total VEGF and the isoforms 121,165 and 189 were all increased at 2h after exercise but with no further increase at 6h. The VEGFR2 was increased at 6h after exercise. For all measured factors, the mRNA increase was greater after exercise with reduced blood flow compared to exercise under normal blood flow condition.The present data with an increase in both the VEGF and VEGFR2 mRNA supports the hypothesis that the VEGF-system is involved in exercise-induced angiogenesis. Furthermore, an increase of all the major VEGF isoforms occurs in response to exercise. Additionally, the greater mRNA increase in response to exercise with reduced blood flow indicate ischemia and/or related metabolic events as possible regulating stimuli.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 036023698

Download citation: RISBibTeXText


Related references

Sulfated fucoidan FP08S2 inhibits lung cancer cell growth in vivo by disrupting angiogenesis via targeting VEGFR2/VEGF and blocking VEGFR2/Erk/VEGF signaling. Cancer Letters 382(1): 44-52, 2016

Isolation and characterization of neutralizing human anti-VEGF scFv antibody that specifically blocks VEGF from binding to VEGFR2. Proceedings of the American Association for Cancer Research Annual Meeting 44: 1292, 2003

Correlation of VEGF and VEGFR2 expression with anti-tumor efficacy of VEGFR2 tyrosine kinase inhibitors and an anti-VEGF monoclonal antibody in xenografts. Proceedings of the American Association for Cancer Research Annual Meeting 44: 933, 2003

Activated forms of VEGF-C and VEGF-D provide improved vascular function in skeletal muscle. Circulation Research 104(11): 1302-1312, 2009

Interactions of VEGF isoforms with VEGFR-1, VEGFR-2, and neuropilin in vivo: a computational model of human skeletal muscle. American Journal of Physiology. Heart and Circulatory Physiology 292(1): H459-H474, 2006

Molecular Pharmacology of VEGF-A Isoforms: Binding and Signalling at VEGFR2. International Journal of Molecular Sciences 19(4), 2018

VEGF(165)b, an antiangiogenic VEGF-A isoform, binds and inhibits bevacizumab treatment in experimental colorectal carcinoma: balance of pro- and antiangiogenic VEGF-A isoforms has implications for therapy. British Journal of Cancer 98(8): 1366-1379, 2008

VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysis. Biology Open 5(5): 571-583, 2016

Tumor-specific VEGF-A and VEGFR2 in postmenopausal breast cancer patients with long-term follow-up. Implication of a link between VEGF pathway and tamoxifen response. Breast Cancer Research and Treatment 89(2): 135-143, 2005

Disease-specific expression of VEGF and its receptors in AML cells: possible autocrine pathway of VEGF/type1 receptor of VEGF in t(15;17) AML and VEGF/type2 receptor of VEGF in t(8;21) AML. Leukemia and Lymphoma 47(1): 89-95, 2006

Real-Time Ligand Binding of Fluorescent VEGF-A Isoforms that Discriminate between VEGFR2 and NRP1 in Living Cells. Cell Chemical Biology 25(10): 1208-1218.E5, 2018

Contracting skeletal muscle cells express and release VEGF and induce VEGF mRNA in endothelial cells. Medicine & Science in Sports & Exercise 33(5 Supplement): S53, 2001

Expression of vascular endothelial growth factors (VEGF-A/VEGF-1 and VEGF-C/VEGF-2) in postmenopausal uterine endometrial carcinoma. Gynecologic Oncology 80(2): 181-188, 2001

The angiogenic switch for vascular endothelial growth factor (VEGF)-A, VEGF-B, VEGF-C, and VEGF-D in the adenoma-carcinoma sequence during colorectal cancer progression. Journal of Pathology 200(2): 183-194, 2003

Circulating levels of VEGF-A, VEGF-D and soluble VEGF-A receptor (sFIt-1) in human breast cancer. International Journal of Biological Markers 19(3): 229-235, 2004