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Manipulation of ploidy for kiwifruit breeding in vitro chromosome doubling in diploid Actinidia chinensis Planch

Coimbra, Joao Carlos; Pinto, Iraja Damiani; Wurdig, Norma Luiza; Do Carmo, Dermeval Aparecido

Plant Cell, Tissue and Organ Culture 106(3): 503-511

2011


ISSN/ISBN: 0167-6857
DOI: 10.1007/s11240-011-9949-z
Accession: 036063361

Tetraploid plants were induced by colchicine treatment of in vitro leaf petiole segment cultures of five diploid Actinidia chinensis Planch. genotypes, including the commercially important, yellow-fleshed cultivar Hort16A , three female selections with red-fleshed fruit and one male pollinizer. Petiole segments were incubated on a shoot regeneration medium for a period of 4 weeks, and subsequently microshoots were treated with .5 or .1% colchicine. About one-third of the regenerated shoots were tetraploid following .5% colchicine treatment, more than with .1% colchicine treatment. Similar rates of tetraploid induction were achieved with all the genotypes tested. The efficiency of induction of polyploidy depended on the interaction between the types of in vitro culture chosen and the concentration of colchicine used. There are no previous reports of colchicine being used so successfully to induce polyploidy in Actinidia..

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