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A Novel E coli Solubility Enhancer Protein for Fusion Expression of Aggregation-prone Heterologous Proteins


A Novel E coli Solubility Enhancer Protein for Fusion Expression of Aggregation-prone Heterologous Proteins







Through the proteome analysis of E. coli BL21(DE3), we previously identified the stress-responsive protein, arsenate reductase (ArsC), that showed a high cytoplasmic solubility and a folding capacity even in the presence of stress-inducing reagents. In this study, we used ArsC as an N-terminal fusion partner to synthesize nine aggregation-prone proteins as water-soluble forms. As a result, solubility of the aggregation-prone proteins increased dramatically by the fusion of ArsC, due presumably to its tendency to facilitate the folding of target proteins. Also, we evaluated and confirmed the efficacy of ArsC-fusion expression in making the fusion-expressed target proteins have their own native.

Accession: 036072395

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