Molecular cloning and expression analysis of the gene encoding sedoheptulose-1, 7-bisphosphatase from Cucumis sativus

Meiling Wang; Huangai Bi; Peipei Liu; Xizhen Ai

Scientia Horticulturae 129(3): 0-420

2011


ISSN/ISBN: 0304-4238
DOI: 10.1016/j.scienta.2011.04.010
Accession: 036095687

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Abstract
Sedoheptulose-1, 7-bisphosphatase (SBPase; EC 3.1.3.37) is a key enzyme in the photosynthetic carbon reduction cycle (Calvin cycle). In the present study, a full-length cDNA encoding SBPase (designated as CsSBP, GenBank accession no. FJ911553) was isolated from cucumber (Cucumis sativus L. cv. Jinyou 3 ) grown in solar-greenhouse by RT-PCR and RACE. The cDNA contained 1452 nucleotides with a complete open reading frame (ORF) of 1167 nucleotides, which was deduced for encoding a peptide of 388 amino acids whose molecular mass was inferred to be 42kDa with its isoelectric point at 6.17. Sequence comparison analysis showed that the deduced amino acid of. Highlights: SBPase gene (CsSBP) in cucumber was first cloned and analyzed. CsSBP expresses only in green tissues of cucumber. There is close correlation between SBPase activity and CsSBP expression. There is close correlation between SBPase activity and the net photosynthetic rate.