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In silico comparative study of the genomic islands of Vibrio cholerae MJ1236 with those of Classical and El Tor N16961 strains of Vibrio cholerae

Dutta, A.; Kundu, J.Kumar.; Chatterjee, R.; Chaudhuri, K.

FEMS microbiology letters 321(1): 75-81

2011


ISSN/ISBN: 0378-1097
PMID: 21599729
DOI: 10.1111/j.1574-6968.2011.02316.x
Accession: 036099098

The evolution of microbial genomes is greatly influenced by horizontal gene transfer (HGT), where large blocks of horizontally acquired foreign sequences often encoding virulence determinants, occur in chromosomes of pathogenic bacteria. A program Design-Island developed in our laboratory was used on three completely sequenced Vibrio cholerae genomes, V. cholerae Classical O395, El Tor N16961 and MJ1236 in order to identify the putative horizontally acquired regions. The putative genomic islands (GIs) were graphically represented and analyzed. The study identified distinct regions in the GIs of V. cholerae MJ1236 which were shared either with the Classical or the El Tor strain of V. cholerae. A cluster comprising of 38 Open Reading Frames (ORFs) were common to V. cholerae strains of MJ1236 and Classical O395 but absent in El Tor N1696About 5% of the predicted GIs of V. cholerae MJ1236 were unique to itself. Amongst these unique ORFs, a region of mostly hypothetical genes was identified, where the ORFs were present in a large cluster. The result show that the HGT had played a significant role in the evolution and the differentiation of the V. cholerae MJ1236.

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