+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Regulation of pituitary inhibin/activin subunits and follistatin gene expression by GnRH in female rats



Regulation of pituitary inhibin/activin subunits and follistatin gene expression by GnRH in female rats



Journal of Endocrinology 210(1): 71-79



Pituitary inhibin B, activin B, and follistatin are local regulators of FSH. Activin B is a homodimeric molecule (β(B)-β(B)), while inhibin B contains an α and a β(B) subunit. The regulation of gene expression of α, β(B), and follistatin by local and endocrine hormones was examined in pituitaries from female rats and in perifused pituitary cells by RT-PCR. Ovariectomy (OVX) induced an elevation in the mRNA level of α and β(B) subunits and follistatin. Short-term (4 h) treatment of pituitary cells with GnRH decreased both the inhibin α and the inhibin/activin β(B) subunit mRNA levels, while long-term treatment (20 h) with 100 nM GnRH stimulated the expression of both subunits. In contrast, the mRNA level of follistatin was elevated after the short-term GnRH treatment. Long-term exposure of pituitary cells to estradiol and inhibin B suppressed the mRNA expression of β(B) and had no effect on the expression of α subunit and follistatin. Our results demonstrate that the increased expressions of inhibin/activin subunits and follistatin in the post-OVX period can be induced by the lack of gonadal negative feedback, resulting in a high GnRH environment in the pituitary. This study reports for the first time that GnRH administered in high doses and for a long period stimulates the gene expression of inhibin/activin subunits and thereby may contribute to the stimulatory effect of OVX on the expression of these genes.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 036119113

Download citation: RISBibTeXText

PMID: 21490134

DOI: 10.1530/joe-10-0485


Related references

Regulation of pituitary follistatin and inhibin/activin subunit messenger ribonucleic acids (mRNAs) in male and female rats: evidence for inhibin regulation of follistatin mRNA in females. Endocrinology 139(6): 2818-2823, 1998

Pituitary activin receptor subtypes and follistatin gene expression in female rats: differential regulation by activin and follistatin. Endocrinology 137(2): 548-554, 1996

Regulation of inhibin/activin subunits and follistatin mRNA expression in the rat pituitary at early estrus. Life Sciences 67(21): 2549-2562, 2000

Expression of inhibin subunits and follistatin during postimplantation mouse development: decidual expression of activin and expression of follistatin in primitive streak, somites and hindbrain. Development 120(4): 803-813, 1994

Pituitary follistatin gene expression in female rats: evidence that inhibin regulates transcription. Biology of Reproduction 70(2): 364-370, 2004

Activin subunit, follistatin, and activin receptor gene expression in the prepubertal female rat pituitary. Biology of Reproduction 59(2): 278-283, 1998

Gonadotropin-releasing hormone regulation of gonadotropin subunit gene expression in female rats Actions on follicle-stimulating hormone beta messenger ribonucleic acid involve differential expression of pituitary activin and follistatin mRNAs. Endocrinology 140(2): 903-908, 1999

Autocrine/paracrine regulation of pituitary function by activin, inhibin and follistatin. Molecular and Cellular Endocrinology 225(1-2): 29-36, 2004

Expression of mRNA for follistatin and inhibin/activin subunits during follicular growth and atresia. Journal of Molecular Endocrinology 13(3): 253-264, 1994

Inhibin, activin, follistatin, activin receptors and beta-glycan gene expression in the villous tissue of miscarriage patients. Molecular Human Reproduction 10(11): 793-798, 2004

Inhibin, activin, follistatin, activin receptors and beta-glycan gene expression in the placental tissue of patients with pre-eclampsia. Molecular Human Reproduction 9(4): 199-203, 2003

Testicular expression of inhibin and activin subunits and follistatin in the rat and human fetus and neonate and during postnatal development in the rat. Endocrinology 138(5): 2136-2147, 1997

Expression of activin/inhibin subunits and follistatin in normal endocrine cells and related tumors of the gut and pancreas. Laboratory Investigation 81(1): 77A, 2001

Expression of follistatin mRNA and inhibin/activin subunits mRNAs and proteins during follicular development in sheep. Journal of Reproduction & Fertility Abstract Series 0(13): 10, 1994

Increased follistatin (activin-binding protein) gene expression in rat anterior pituitary tissue after ovariectomy may be mediated by pituitary activin. Endocrinology 132(5): 2221-2228, 1993