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Detection of oral squamous cell carcinoma and cervical lymph node metastasis using activatable near-infrared fluorescence agents



Detection of oral squamous cell carcinoma and cervical lymph node metastasis using activatable near-infrared fluorescence agents



Archives of Otolaryngology--Head & Neck Surgery 137(6): 609-615



To assess the feasibility of optical imaging using activatable near-infrared fluorescence (NIRF) agents to detect oral cancer and cervical lymph node metastasis in vivo. Design In vivo study. University medical center.Subjects Female nude mice aged 4 to 6 weeks.Intervention Luciferase-expressing OSC-19-luc cells were injected into the tongues of nude mice. A control group of nude mice was injected in the tongue with a physiologic saline solution. Tumor growth was followed by bioluminescence imaging. After 3 weeks, animals were randomly allocated to intravenous administration of 1 of 2 activatable NIRF agents: ProSense68 or MMPSense68. Fluorescence imaging of the mice was performed, and the tumor to background ratio (TBR) was determined on histologic sections of the tongue and cervical lymph nodes after resection at necropsy.Main Outcome Measure Fluorescence signals. The fluorescence signals in tongue tumor and cervical lymph node metastases were significantly higher than those in control animals. The mean (SD) TBR of ProSense68 in the tongue was 15.8 (8.1) and in the lymph nodes was 11.8 (3.6). For MMPSense68, the mean (SD) TBR in the tongue was 18.6 (9.4) and in the lymph nodes was 1.5 (4.). Oral cancer and cervical lymph node metastases can be detected by targeting increased proteolytic activity at the tumor borders using NIRF optical imaging. These NIRF agents could be used for real-time image-guided surgery, which has the potential to improve the complete surgical resection of oral cancer.

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Accession: 036127618

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PMID: 21690514

DOI: 10.1001/archoto.2011.89


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