+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

An efficient Agrobacterium-mediated transformation and regeneration system for leaf explants of two elite aspen hybrid clones Populus alba × P berolinensis and Populus davidiana × P bolleana

An efficient Agrobacterium-mediated transformation and regeneration system for leaf explants of two elite aspen hybrid clones Populus alba × P berolinensis and Populus davidiana × P bolleana

Transgenic technology has been successfully used for gene function analyses and trait improvement in cereal plants. However, its usage is limited in woody plants, especially in the difficult-to-transform but commercially viable hybrid poplar. In this work, an efficient regeneration and transformation system was established for the production of two hybrid aspen clones: Populus alba P. berolinensis and Populus davidiana P. bolleana. A plant transformation vector designed to express the reporter gene uidA, encoding ?-glucuronidase (GUS), driven by the cauliflower mosaic virus 35S promoter, was used to detect transformation event at early stages of plant regeneration, and to optimize the parameters that may affect poplar transformation efficiency. Bacterium strain and age of leaf explant are two major factors that affect transformation efficiency. Addition of thidiazuron (TDZ) improved both regeneration and transformation efficiency. The transformation efficiency is approximately 9.3% for P. alba P. berolinensis and 16.4% for P. davidiana P. bolleana. Using this system, transgenic plants were usually produced in less than 1 month after co-cultivation. The growth characteristics and morphology of transgenic plants were identical to the untransformed wild type plants, and the transgenes could be inherited by vegetative propagation, as confirmed by PCR, Southern blotting, RT PCR and ?-glucuronidase staining analyses. The establishment of this system will help to facilitate the studies of gene functions in tree growth and development at a genome level, and as well as the introduction of some valuable traits in aspen breeding.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 036138447

Download citation: RISBibTeXText

Related references

An efficient Agrobacterium-mediated transformation and regeneration system for leaf explants of two elite aspen hybrid clones Populus alba × P. berolinensis and Populus davidiana × P. bolleana. Plant Cell Reports 30(11): 2037-2044, 2012

Efficient Agrobacterium-Mediated Transformation of Hybrid Poplar Populus davidiana Dode x Populus bollena Lauche. International Journal of Molecular Sciences 14(2): 2515-2528, 2013

In vitro transformation of aspen (Populus tremula X Populus alba ) by Agrobacterium rhizogenes and regeneration of plants resistant to Basta. Comptes Rendus de l' Academie des Sciences Series 3, Sciences de la Vie 314(6): 291-298, 1992

Genetic transformation of aspen populus tremula x populus alba by agrobacterium rhizogenes and regeneration of plants tolerant to herbicide. Comptes Rendus de l'Academie des Sciences Serie III Sciences de la Vie 314(6): 291-298K, 1992

Plant regeneration and Agrobacterium-mediated transformation of two elite aspen hybrid clones from in vitro leaf tissues. In Vitro Cellular and Developmental Biology Plant 39(1): 6-11, 2003

Plant Regeneration and Agrobacterium-Mediated Transformation of Two Elite Aspen Hybrid Clones from In vitro Leaf Tissues. In Vitro Cellular & Developmental Biology. Plant 39(1): 6-11, 2003

Studies on optimum site selection methods for the new hybrid poplars populus alba x populus glandulosa f 1 clones populus koreana x populus nigra var italica f 1 clones and populus nigra x populus maximowiczii f 1 clones. Research Report of the Forest Genetics Research Institute (21): 37-52, 1985

Agrobacterium tumefaciens-mediated transformation of elite white poplar (Populus alba L.) and regeneration of transgenic plants. Journal of Genetics and Breeding 54(4): 263-270, 2000

Organogenesis of leaf explant of populus davidiana x populus bolleana hybrid and effect of growth regulators on organogenesis. Acta Botanica Sinica 22(4): 311-315, 1980

Establishment of a highly efficient plant regeneration system of Populus davidiana x P bolleana and study of its genetic stability. Journal Of Beijing Forestry University: 3, 68-73, 2008

Agrobacterium-mediated transformation of quaking aspen (Populus tremuloides) and regeneration of transgenic plants. Plant Cell Reports 14(2-3): 94-97, 1994

Factors effecting agrobacterium mediated transformation and regeneration of populus nigra x populus maximowiczii. Journal of Korean Forestry Society 79(3): 278-284, 1990

Efficient shoot regeneration from internodal explants of Populus angustifolia, Populus balsamifera and Populus deltoids. New Biotechnology 28(6): 778-787, 2012

Differences in leaf characteristics between ozone-sensitive and ozone-tolerant hybrid aspen (Populus tremula x Populus tremuloides) clones. Tree Physiology 29(1): 53-66, 2009

Efficacy of Cry1Ac protein against gypsy moth and fall webworm in transgenic poplar (Populus davidiana x Populus bolleana) by bioassay. Canadian Journal of Plant Science 98(4): 844-850, 2018